caa a22 4500 445318201 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9375-6 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9375-6 Functional Divergences of GAPDH Isoforms During Early Development in Two Perciform Fish Species [Elektronische Daten] [Elena Sarropoulou, Dimitra Nousdili, Georgios Kotoulas, Antonios Magoulas] Glyceraldehyde-3-phospate dehydrogenase (GAPDH) is involved in basic cell catabolic processes and, as it is thought to be continuously expressed, belongs to the group of housekeeping genes. Thus, it is frequently used as an internal control in quantitative gene expression studies. However, the evidence of different expression patterns in a broad range of organisms and tissues, as well as the occurrence of different isoforms, shows that GAPDH has to be reevaluated as an internal control in qPCR studies, and its annotation has to be enriched. GAPDH has been shown to be involved in the pathway of energy and carbon molecule supply as well as in transcription and apoptosis. In the present study, we isolated the two isoforms, GAPDH-1 and GAPDH-2, of the gilthead sea bream (Sparus aurata) and the European sea bass (Dicentrarchus labrax). We inferred the phylogenetic relationships to ten other fish species and gave the gene structure of both genes. We further investigated gene expression analysis in both species for different developmental stages showing divergent gene expression of the two isoforms and the possible function of GAPDH-1 as a maternal gene. Springer Science+Business Media, LLC, 2011 GAPDH nationallicence Isoforms nationallicence Development nationallicence Differential expression nationallicence Glycolytic pathway nationallicence Perciformes nationallicence Sarropoulou Elena Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut Nousdili Dimitra Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut Kotoulas Georgios Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut Magoulas Antonios Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1115-1124 1436-2228 13:6<1115 2011 13 10126 https://doi.org/10.1007/s10126-011-9375-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9375-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sarropoulou Elena Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut NATIONALLICENCE 700 1- Nousdili Dimitra Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut NATIONALLICENCE 700 1- Kotoulas Georgios Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut NATIONALLICENCE 700 1- Magoulas Antonios Institute of Marine Biology and Genetics (I.M.B.G.), Hellenic Centre for Marine Research Crete, Gournes Pediados, P.O. Box2214, 71003, Heraklion, Crete, Greece aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1115-1124 1436-2228 13:6<1115 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer