caa a22 4500 445318236 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10126-011-9379-2 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9379-2 Novel Proteins from the Calcifying Shell Matrix of the Pacific Oyster Crassostrea gigas [Elektronische Daten] [Benjamin Marie, Isabelle Zanella-Cléon, Nathalie Guichard, Michel Becchi, Frédéric Marin] The shell of the Pacific oyster Crassostrea gigas is composed of more than 99% CaCO3 and of around 0.5% of occluded organic matrix. According to classical views, this matrix is supposed to regulate the shell mineral deposition. In this study, we developed one of the first proteomic approaches applied to mollusk shell in order to characterise the calcifying matrix proteins. The insoluble organic matrix, purified after demineralisation of the shell powder, was digested with trypsin enzyme, and separated on nano-LC, prior to nanospray quadrupole/time-of-flight analysis. MS/MS spectra were searched against the above 220,000 EST sequences available in the public database for Crassostrea. Using this approach, we were able to identify partial or full-length sequence transcripts that encode eight novel shell matrix proteins. Springer Science+Business Media, LLC, 2011 Biomineralization nationallicence Calcium carbonate nationallicence EGF-like domain nationallicence Foliated calcite nationallicence Mollusk shell nationallicence Organic matrix nationallicence Proteomics nationallicence AA : Amino acid nationallicence ASM : Acid-soluble matrix nationallicence AIM : Acid-insoluble matrix nationallicence EGF : Epidermal growth factor nationallicence IMSP : Insoluble matrix shell protein nationallicence SEM : Scanning electron microscopy nationallicence EST : Expressed sequence tag nationallicence Marie Benjamin UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 Bd. Gabriel, 21000, Dijon, France aut Zanella-Cléon Isabelle IFR 128 BioSciences; UMR 5086 CNRS; IBCP, Université de Lyon 1, Lyon, France aut Guichard Nathalie UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 Bd. Gabriel, 21000, Dijon, France aut Becchi Michel IFR 128 BioSciences; UMR 5086 CNRS; IBCP, Université de Lyon 1, Lyon, France aut Marin Frédéric UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 Bd. Gabriel, 21000, Dijon, France aut Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1159-1168 1436-2228 13:6<1159 2011 13 10126 https://doi.org/10.1007/s10126-011-9379-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9379-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marie Benjamin UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 Bd. Gabriel, 21000, Dijon, France aut NATIONALLICENCE 700 1- Zanella-Cléon Isabelle IFR 128 BioSciences; UMR 5086 CNRS; IBCP, Université de Lyon 1, Lyon, France aut NATIONALLICENCE 700 1- Guichard Nathalie UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 Bd. Gabriel, 21000, Dijon, France aut NATIONALLICENCE 700 1- Becchi Michel IFR 128 BioSciences; UMR 5086 CNRS; IBCP, Université de Lyon 1, Lyon, France aut NATIONALLICENCE 700 1- Marin Frédéric UMR 5561 CNRS Biogéosciences, Université de Bourgogne, 6 Bd. Gabriel, 21000, Dijon, France aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/6(2011-12-01), 1159-1168 1436-2228 13:6<1159 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer