caa a22 4500 445318252 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9323-x doi (NATIONALLICENCE)springer-10.1007/s10126-010-9323-x Stress-Induced Changes in Optical Properties, Pigment and Fatty Acid Content of Nannochloropsis sp.: Implications for Non-destructive Assay of Total Fatty Acids [Elektronische Daten] [Alexei Solovchenko, Inna Khozin-Goldberg, Lee Recht, Sammy Boussiba] In order to develop a practical approach for fast and non-destructive assay of total fatty acid (TFA) and pigments in the biomass of the marine microalga Nannochloropsis sp. changes in TFA, chlorophyll, and carotenoid contents were monitored in parallel with the cell suspension absorbance. The experiments were conducted with the cultures grown under normal (complete nutrient f/2 medium at 75μmol PAR photons/(m2 s)) or stressful (nitrogen-lacking media at 350 μmol PAR photons/(m2 s)) conditions. The reliable measurement of the cell suspension absorbance using a spectrophotometer without integrating sphere was achieved by deposition of cells on glass-fiber filters in the chlorophyll content range of 3-13mg/L. Under stressful conditions, a 30-50% decline in biomass and chlorophyll, retention of carotenoids and a build-up of TFA (15-45 % of dry weight) were recorded. Spectral regions sensitive to widely ranging changes in carotenoid-to-chlorophyll ratio and correlated changes of TFA content were revealed. Employing the tight inter-correlation of stress-induced changes in lipid metabolism and rearrangement of the pigment apparatus, the spectral indices were constructed for non-destructive assessment of carotenoid-to-chlorophyll ratio (range 0.3-0.6; root mean square error (RMSE) = 0.03; r 2 = 0.93) as well as TFA content of Nannochloropsis sp. biomass (range 5.0-45%; RMSE = 3.23 %; r 2 = 0.89) in the broad band 400-550nm normalized to that in chlorophyll absorption band (centered at 678nm). The findings are discussed in the context of real-time monitoring of the TFA accumulation by Nannochloropsis cultures under stressful conditions. Springer Science+Business Media, LLC, 2010 Carotenoids nationallicence Microalgal biotechnology nationallicence Nannochloropsis nationallicence Photoadaptation nationallicence Non-destructive assay nationallicence Car : Carotenoid(s) nationallicence Chl : Chlorophyll(s) nationallicence DW : Biomass dry weight nationallicence IS : Integrating sphere nationallicence PFD : Photon Flux Density nationallicence (T)FA : (Total) fatty acids nationallicence Solovchenko Alexei Department of Biotechnology, Faculty of Biology, Moscow State University, GSP-1, 119991, Moscow, Russia aut Khozin-Goldberg Inna The Microalgal Biotechnology Laboratory, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, 84990, Midreshet Ben-Gurion, Israel aut Recht Lee The Microalgal Biotechnology Laboratory, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, 84990, Midreshet Ben-Gurion, Israel aut Boussiba Sammy The Microalgal Biotechnology Laboratory, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, 84990, Midreshet Ben-Gurion, Israel aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 527-535 1436-2228 13:3<527 2011 13 10126 https://doi.org/10.1007/s10126-010-9323-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9323-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Solovchenko Alexei Department of Biotechnology, Faculty of Biology, Moscow State University, GSP-1, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Khozin-Goldberg Inna The Microalgal Biotechnology Laboratory, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, 84990, Midreshet Ben-Gurion, Israel aut NATIONALLICENCE 700 1- Recht Lee The Microalgal Biotechnology Laboratory, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, 84990, Midreshet Ben-Gurion, Israel aut NATIONALLICENCE 700 1- Boussiba Sammy The Microalgal Biotechnology Laboratory, The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boker Campus, 84990, Midreshet Ben-Gurion, Israel aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 527-535 1436-2228 13:3<527 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer