caa a22 4500 445318260 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9309-8 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9309-8 A Novel Omega-3 Fatty Acid Desaturase Involved in Acclimation Processes of Polar Condition from Antarctic Ice Algae Chlamydomonas sp. ICE-L [Elektronische Daten] [Pengying Zhang, Shenghao Liu, Bailin Cong, Guangting Wu, Chenlin Liu, Xuezheng Lin, Jihong Shen, Xiaohang Huang] The ability of Antarctic ice algae, Chlamydomonas sp. ICE-L, to survive and proliferate at low temperature and high salinity implies that they have overcome key barriers inherent in Antarctic environments. A full-length complementary DNA (cDNA) sequence of omega-3 fatty acid desaturase, designated CiFAD3, was isolated via reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The full-length of CiFAD3 cDNA contained an open reading frame of 1,302bp with 5′-terminal untranslated region (UTR) of 36bp and 3′-terminal UTR of 507bp encoding a fatty acid desaturase protein of 434 amino acids. Sequence alignment and phylogenetic analysis showed that the gene was homologous to known chloroplastic omega-3 fatty acid desaturase. Meanwhile, CiFAD3 sequence showed typical features of membrane-bound desaturase such as three conserved histidine boxes along with four membrane spanning regions that were universally present among plant desaturases. Under different stress conditions, messenger RNA (mRNA) expression levels of CiFAD3 were measured by quantitative RT-PCR. The results showed that both temperature and salinity could motivate the upregulation of CiFAD3 expression. The mRNA accumulation of CiFAD3 increased 2.6-fold at 0°C and 1.8-fold at 12°C compared to the algae at 6°C. Similarly, mRNA expression levels of CiFAD3 increased 3.8-fold after 62‰ NaCl treatment for 2h. However, CiFAD3 mRNA expression levels were partially decreased after UV radiation. These data suggest that CiFAD3 is the enzyme responsible for the omega-3 fatty acid desaturation involved in ice algae Chlamydomonas sp. ICE-L acclimatizing to cold temperature and high salinity in Antarctic environment. Springer Science+Business Media, LLC, 2010 Antarctic ice algae nationallicence Chlamydomonas sp. ICE-L nationallicence Omega-3 fatty acid desaturase nationallicence Psychrophile nationallicence Stress nationallicence Zhang Pengying National Glycoengineering Research Center and College of Life Science, Shandong University, 250100, Jinan, People's Republic of China aut Liu Shenghao Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut Cong Bailin Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut Wu Guangting Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut Liu Chenlin Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut Lin Xuezheng Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut Shen Jihong Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut Huang Xiaohang Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 393-401 1436-2228 13:3<393 2011 13 10126 https://doi.org/10.1007/s10126-010-9309-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9309-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Pengying National Glycoengineering Research Center and College of Life Science, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Liu Shenghao Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Cong Bailin Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Wu Guangting Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Liu Chenlin Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Lin Xuezheng Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Shen Jihong Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Huang Xiaohang Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, 266061, Qingdao, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 393-401 1436-2228 13:3<393 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer