caa a22 4500 445318287 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9319-6 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9319-6 Characterisation of the Carbohydrate Fraction of the Temporary Adhesive Secreted by the Tube Feet of the Sea Star Asterias rubens [Elektronische Daten] [Elise Hennebert, Ruddy Wattiez, Patrick Flammang] In sea stars, adhesion takes place at the level of a multitude of small appendages, the tube feet. It involves the secretion of an adhesive material which, after tube foot detachment, remains on the substratum as a footprint. It was previously reported that the two main organic components of this material are proteins and carbohydrates. The carbohydrate moiety of the adhesive secretion of Asterias rubens was investigated using a set of 16 lectins which were used on sections through tube feet, on footprints, and on the proteins extracted from these footprints. After gel electrophoresis, these proteins separate into eight protein bands which were named sea star footprint proteins (Sfps). Eleven lectins label the tube foot epidermis at the level of the adhesive cells, four react with footprints, and eight with two of the extracted footprint proteins, which are therefore classified as glycoproteins. Sfp-290 appears to bear mostly N-linked oligosaccharides and Sfp-210 principally O-linked oligosaccharides. The outer chains of both glycoproteins enclose galactose, N-acetylgalactosamine, fucose, and sialic acid residues. Another part of the carbohydrate fraction of the footprints would be in the form of larger molecules, such as sialylated proteoglycans. These two types of glycoconjugates are presumably key components of the sea star temporary adhesive providing both cohesive and adhesive contributions through electrostatic interactions by the polar and hydrogen-bonding functional groups of their glycan chains. Springer Science+Business Media, LLC, 2010 Marine adhesion nationallicence Footprints nationallicence Glycoproteins nationallicence Lectins nationallicence Echinodermata nationallicence Hennebert Elise Laboratoire de Biologie Marine, Université de Mons—UMONS, Pentagone 2B 6, Avenue du Champ de Mars, 7000, Mons, Belgium aut Wattiez Ruddy Laboratoire de Protéomie et de Microbiologie, Université de Mons—UMONS, Mons, Belgium aut Flammang Patrick Laboratoire de Biologie Marine, Université de Mons—UMONS, Pentagone 2B 6, Avenue du Champ de Mars, 7000, Mons, Belgium aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 484-495 1436-2228 13:3<484 2011 13 10126 https://doi.org/10.1007/s10126-010-9319-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9319-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hennebert Elise Laboratoire de Biologie Marine, Université de Mons—UMONS, Pentagone 2B 6, Avenue du Champ de Mars, 7000, Mons, Belgium aut NATIONALLICENCE 700 1- Wattiez Ruddy Laboratoire de Protéomie et de Microbiologie, Université de Mons—UMONS, Mons, Belgium aut NATIONALLICENCE 700 1- Flammang Patrick Laboratoire de Biologie Marine, Université de Mons—UMONS, Pentagone 2B 6, Avenue du Champ de Mars, 7000, Mons, Belgium aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 484-495 1436-2228 13:3<484 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer