caa a22 4500 445318309 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9308-9 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9308-9 Validation of Housekeeping Genes for Gene Expression Studies in Symbiodinium Exposed to Thermal and Light Stress [Elektronische Daten] [Nedeljka Rosic, Mathieu Pernice, Mauricio Rodriguez-Lanetty, Ove Hoegh-Guldberg] Unicellular photosynthetic algae (dinoflagellate) from the genus Symbiodinium live in mutualistic symbiosis with reef-building corals. Cultured Symbiodinium sp. (clade C) were exposed to a range of environmental stresses that included elevated temperatures (29°C and 32°C) under high (100μmol quanta m−2s−1 Photosynthetic Active Radiation) and low (10μmol quanta m−2s−1) irradiances. Using real-time RT-PCR the stability of expression for the nine selected putative housekeeping genes (HKGs) was tested. The most stable expression pattern was identified for cyclophilin and S-adenosyl methionine synthetase (SAM) followed by S4 ribosomal protein (Rp-S4), Calmodulin (Cal), and Cytochrome oxidase subunit 1 (Cox), respectively. Thermal stress alone resulted in the highest expression stability for Rp-S4 and SAM, with a minimum of two reference genes required for data normalization. For Symbiodinium exposed to both, light and thermal stresses, at least five reference genes were recommended by geNorm analysis. In parallel, the expression of Hsp90 for Symbiodinium in culture and in symbiosis within coral host (Acropora millepora) was evaluated using the most stable HKGs. Our results revealed a drop in Hsp90 expression after an 18 h-period and a 24 h-period of exposure to elevated temperatures indicating the similar Hsp90 expression profile in symbiotic and non-symbiotic environments. This study provides the first list of the HKGs and will provide a useful reference in future gene expression studies in symbiotic dinoflagellates. Springer Science+Business Media, LLC, 2010 Housekeeping genes nationallicence Real-time RT-PCR nationallicence Gene expression nationallicence Dinoflagellate nationallicence Climate change nationallicence Rosic Nedeljka Global Change Institute, University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia aut Pernice Mathieu Global Change Institute, University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia aut Rodriguez-Lanetty Mauricio Department of Biology, University of Louisiana at Lafayette, 70504, Lafayette, LA, USA aut Hoegh-Guldberg Ove Global Change Institute, University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 355-365 1436-2228 13:3<355 2011 13 10126 https://doi.org/10.1007/s10126-010-9308-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9308-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rosic Nedeljka Global Change Institute, University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Pernice Mathieu Global Change Institute, University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Rodriguez-Lanetty Mauricio Department of Biology, University of Louisiana at Lafayette, 70504, Lafayette, LA, USA aut NATIONALLICENCE 700 1- Hoegh-Guldberg Ove Global Change Institute, University of Queensland, St. Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 355-365 1436-2228 13:3<355 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer