caa a22 4500 445318317 CHVBK 20180317142654.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9315-x doi (NATIONALLICENCE)springer-10.1007/s10126-010-9315-x Lipovitellin is a Non-Self Recognition Receptor with Opsonic Activity [Elektronische Daten] [Jun Zhang, Shicui Zhang] Lipovitellin (Lv), a glycolipoprotein, is a major component of the egg yolk, which is usually regarded as an energy reserve of nutrients essential for growth and development. We have purified Lv from ovulated eggs of the rosy barb Puntius conchonius by two-step chromatography and characterized it by staining with periodic acid/Schiff reagent and Sudan black B, amino acid composition analysis, and peptide mass fingerprinting. The results of ligand and bacterial binding assays, an enzyme-linked immunosorbent assay (ELISA), and the phagocytosis test revealed, for the first time, that the purified native form of P. conchonius Lv acts as a pattern recognition molecule with multiple specificities capable of identifying pathogen-associated molecular patterns (PAMPs), including those of lipopolysaccharide, lipoteichoic acid, and peptidoglycan, rather than self components and that it can bind Gram-negative and -positive bacteria, such as Escherichia coli and Staphylococcus aureus. These tests also showed that the P. conchonius Lv functions as an opsonin capable of enhancing macrophage phagocytosis. Taken together, these characteristics suggest that in developing embryos/larvae of P. conchonius, the native form of Lv may be physiologically involved in the sensing of invading pathogens via interaction with PAMPs and in the recruitment of the primitive macrophages that appear in early embryos to phagocytose and digest the pathogens, thereby protecting them from pathogenic attacks. Springer Science+Business Media, LLC, 2010 Yolk protein nationallicence Lipovitellin nationallicence Pattern recognition receptor nationallicence Opsonin nationallicence Antibacterial activity nationallicence Zhang Jun Department of Marine Biology and Laboratory for Evolution and Development, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Zhang Shicui Department of Marine Biology and Laboratory for Evolution and Development, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 441-450 1436-2228 13:3<441 2011 13 10126 https://doi.org/10.1007/s10126-010-9315-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9315-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Jun Department of Marine Biology and Laboratory for Evolution and Development, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Zhang Shicui Department of Marine Biology and Laboratory for Evolution and Development, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 441-450 1436-2228 13:3<441 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer