caa a22 4500 445318341 CHVBK 20180317142655.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9313-z doi (NATIONALLICENCE)springer-10.1007/s10126-010-9313-z A Novel Method for Coral Explant Culture and Micropropagation [Elektronische Daten] [Maya Vizel, Yossi Loya, Craig Downs, Esti Kramarsky-Winter] We describe here a method for the micropropagation of coral that creates progeny from tissue explants derived from a single polyp or colonial corals. Coral tissue explants of various sizes (0.5-2.5mm in diameter) were manually microdissected from the solitary coral Fungia granulosa. Explants could be maintained in an undeveloped state or induced to develop into polyps by manipulating environmental parameters such as light and temperature regimes, as well as substrate type. Fully developed polyps were able to be maintained for a long-term in a closed sea water system. Further, we demonstrate that mature explants are also amenable to this technique with the micropropagation of second-generation explants and their development into mature polyps. We thereby experimentally have established coral clonal lines that maintain their ability to differentiate without the need for chemical induction or genetic manipulation. The versatility of this method is also demonstrated through its application to two other coral species, the colonial corals Oculina patigonica and Favia favus. Springer Science+Business Media, LLC, 2010 Explant culture nationallicence Clonal lines nationallicence Micropropagation nationallicence Genetically identical nationallicence Vizel Maya Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel aut Loya Yossi Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel aut Downs Craig Haereticus Environmental Laboratory, P.O. Box92, 24533, Clifford, VA, USA aut Kramarsky-Winter Esti Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 423-432 1436-2228 13:3<423 2011 13 10126 https://doi.org/10.1007/s10126-010-9313-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9313-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vizel Maya Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Loya Yossi Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Downs Craig Haereticus Environmental Laboratory, P.O. Box92, 24533, Clifford, VA, USA aut NATIONALLICENCE 700 1- Kramarsky-Winter Esti Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 423-432 1436-2228 13:3<423 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer