caa a22 4500 44531835X CHVBK 20180317142655.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9322-y doi (NATIONALLICENCE)springer-10.1007/s10126-010-9322-y Molecular and Functional Analyses of Aspolin, a Fish-Specific Protein Extremely Rich in Aspartic Acid [Elektronische Daten] [Shigeharu Kinoshita, Eriko Katsumi, Hiroshi Yamamoto, Kazuharu Takeuchi, Shugo Watabe] Aspolin is a muscular protein having unique structural characteristics where the most part of its primary structure is occupied by aspartic acid. Aspolin has been found exceptionally in fish muscle, suggesting its specific role in this tissue. However, biological functions of aspolin have remained unknown. In the present study, we cloned full-length cDNAs encoding zebrafish Danio rerio aspolins 1 and 2, revealed their genomic organization, and examined in vivo function using knockdown techniques. Genomic analysis clearly showed that aspolin is a paralog of the histidine-rich calcium binding protein gene, which encodes a calcium binding protein in sarcoplasmic reticulum (SR). Expression analysis showed that the transcripts and their translated products, aspolins 1 and 2, are distributed in myotomal skeletal muscle, but not in cardiac muscle. Injection of antisense morpholino oligo targeting both aspolins 1 and 2 increased the mRNA levels of calsequestrin 1, another calcium binding protein in SR. These lines of evidence suggest that aspolins regulate calcium concentrations in SR. Springer Science+Business Media, LLC, 2010 Aspolin nationallicence Molecular evolution nationallicence Histidine-rich calcium binding protein (HRC) nationallicence Sarcoplasmic reticulum nationallicence Muscle nationallicence Fish nationallicence Kinoshita Shigeharu Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut Katsumi Eriko Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut Yamamoto Hiroshi Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut Takeuchi Kazuharu Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Kitanomachi, 192-0906, Hachioji, Tokyo, Japan aut Watabe Shugo Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 517-526 1436-2228 13:3<517 2011 13 10126 https://doi.org/10.1007/s10126-010-9322-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9322-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kinoshita Shigeharu Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Katsumi Eriko Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamamoto Hiroshi Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Takeuchi Kazuharu Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Kitanomachi, 192-0906, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Watabe Shugo Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, 113-8657, Bunkyo, Tokyo, Japan aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 517-526 1436-2228 13:3<517 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer