caa a22 4500 445318376 CHVBK 20180317142655.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9317-8 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9317-8 The Protective Effect of ENA Actimineral Resource A on CCl4-Induced Liver Injury in Rats [Elektronische Daten] [Il-Hwa Hong, Hoon Ji, Sung-Yong Hwa, Won-Il Jeong, Da-Hee Jeong, Sun-Hee Do, Ji-Min Kim, Mi-Ran Ki, Jin-Kyu Park, Moon-Jung Goo, Ok-Kyung Hwang, Kyung-Sook Hong, Jung-Youn Han, Hae-Young Chung, Kyu-Shik Jeong] ENA Actimineral Resource A (ENA-A) is alkaline water that is composed of refined edible cuttlefish bone and two different species of seaweed, Phymatolithon calcareum and Lithothamnion corallioides. In the present study, ENA-A was investigated as an antioxidant to protect against CCl4-induced oxidative stress and hepatotoxicity in rats. Liver injury was induced by either subacute or chronic CCl4 administration, and the rats had free access to tap water mixed with 0% (control group) or 10% (v/v) ENA-A for 5 or 8weeks. The results of histological examination and measurement of antioxidant activity showed that the reactive oxygen species production, lipid peroxidation, induction of CYP2E1 were decreased and the antioxidant activity, including glutathione and catalase production, was increased in the ENA-A groups as compared with the control group. On 2-DE gel analysis of the proteomes, 13 differentially expressed proteins were obtained in the ENA-A groups as compared with the control group. Antioxidant proteins, including glutathione S-transferase, kelch-like ECH-associated protein 1, and peroxiredoxin 1, were increased with hepatocyte nuclear factor 3-beta and serum albumin precursor, and kininogen precursor decreased more in the ENA-A groups than compared to the control group. In conclusion, our results suggest that ENA-A does indeed have some protective capabilities against CCl4-induced liver injury through its antioxidant function. Springer Science+Business Media, LLC, 2010 ENA actimineral resource A nationallicence Antioxidant capability nationallicence Hepatoxicity nationallicence Seaweed nationallicence Carbon tetrachloride nationallicence Hong Il-Hwa Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Ji Hoon Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Hwa Sung-Yong Jinju Bio Food, 660-931, Jinju, Republic of Korea aut Jeong Won-Il Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Jeong Da-Hee Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Do Sun-Hee Department of Clinical Pathology,College of Veterinary Medicine, Konkuk University, 143-701, Seoul, Republic of Korea aut Kim Ji-Min Department of Pharmacy, Longevity Life Science and Technology Institute, Pusan National University, 609-735, Pusan, Republic of Korea aut Ki Mi-Ran Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Park Jin-Kyu Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Goo Moon-Jung Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Hwang Ok-Kyung Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Hong Kyung-Sook Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Han Jung-Youn Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Chung Hae-Young Department of Pharmacy, Longevity Life Science and Technology Institute, Pusan National University, 609-735, Pusan, Republic of Korea aut Jeong Kyu-Shik Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 462-473 1436-2228 13:3<462 2011 13 10126 https://doi.org/10.1007/s10126-010-9317-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9317-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hong Il-Hwa Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Ji Hoon Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Hwa Sung-Yong Jinju Bio Food, 660-931, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Jeong Won-Il Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Jeong Da-Hee Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Do Sun-Hee Department of Clinical Pathology,College of Veterinary Medicine, Konkuk University, 143-701, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Kim Ji-Min Department of Pharmacy, Longevity Life Science and Technology Institute, Pusan National University, 609-735, Pusan, Republic of Korea aut NATIONALLICENCE 700 1- Ki Mi-Ran Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Park Jin-Kyu Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Goo Moon-Jung Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Hwang Ok-Kyung Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Hong Kyung-Sook Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Han Jung-Youn Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Chung Hae-Young Department of Pharmacy, Longevity Life Science and Technology Institute, Pusan National University, 609-735, Pusan, Republic of Korea aut NATIONALLICENCE 700 1- Jeong Kyu-Shik Department of Pathology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 462-473 1436-2228 13:3<462 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer