caa a22 4500 445318384 CHVBK 20180317142655.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9325-8 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9325-8 Identification of Copper-Induced Genes in the Marine Alga Ulva compressa (Chlorophyta) [Elektronische Daten] [Loretto Contreras-Porcia, Geraldine Dennett, Alberto González, Eva Vergara, Cristóbal Medina, Juan Correa, Alejandra Moenne] In order to identify genes/proteins involved in copper tolerance, the marine alga Ulva compressa was cultivated with 10μM copper for 3days. The activities of antioxidant enzymes ascorbate peroxidase (AP), peroxiredoxin (PRX), thioredoxin (TRX), and glutathione-S-transferase (GST) and the level of lipoperoxides were determined in the alga cultivated with and without copper addition. Antioxidant enzyme activities and lipoperoxides level increased in response to copper excess, indicating that the alga was under oxidative stress. A cDNA library was prepared using U. compressa cultivated with 10μM copper for 3days. A total of 3 × 104 clones were isolated and 480 clones were sequenced, resulting in 235 non-redundant ESTs, of which 104 encode proteins with known functions. Among them, we identified proteins involved in (1) antioxidant metabolism such as AP, PRX, TRX, GST, and metalothionein (MET), (2) signal transduction, such as calmodulin (CAM), (3) calcium-dependent protein kinase (CDPK) and nucleoside diphosphate kinase (NDK), (4) gene expression, (5) protein synthesis and degradation, and (6) chloroplast and mitochondria electron transport chains. Half of the identified proteins are potentially localized in organelles. The relative level of 18 genes, including those coding for AP, PRX, TRX, GST, MET, CAM, CDPK, and NDK were determined by quantitative RT-PCR in the alga cultivated with 10μM copper for 0 to 7days. Transcript levels increased in response to copper stress and most of them reached a maximum at days3 and 5. Thus, the selected genes are induced by copper stress and they are probably involved in copper acclimation and tolerance. Springer Science+Business Media, LLC, 2010 Antioxidant enzymes nationallicence Lipoperoxides nationallicence cDNA library nationallicence Copper-induced genes nationallicence Marine alga nationallicence Ulva compressa nationallicence Contreras-Porcia Loretto Departamento de Ecología, Center for Advanced Studies in Ecology and Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 6513677, Santiago, Chile aut Dennett Geraldine Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut González Alberto Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut Vergara Eva Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut Medina Cristóbal Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut Correa Juan Departamento de Ecología, Center for Advanced Studies in Ecology and Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 6513677, Santiago, Chile aut Moenne Alejandra Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 544-556 1436-2228 13:3<544 2011 13 10126 https://doi.org/10.1007/s10126-010-9325-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9325-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Contreras-Porcia Loretto Departamento de Ecología, Center for Advanced Studies in Ecology and Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 6513677, Santiago, Chile aut NATIONALLICENCE 700 1- Dennett Geraldine Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut NATIONALLICENCE 700 1- González Alberto Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut NATIONALLICENCE 700 1- Vergara Eva Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut NATIONALLICENCE 700 1- Medina Cristóbal Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut NATIONALLICENCE 700 1- Correa Juan Departamento de Ecología, Center for Advanced Studies in Ecology and Biodiversity, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 6513677, Santiago, Chile aut NATIONALLICENCE 700 1- Moenne Alejandra Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40 correo 33, Santiago, Chile aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 544-556 1436-2228 13:3<544 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer