caa a22 4500 445318406 CHVBK 20180317142655.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9316-9 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9316-9 Isolation and Identification of a Novel Antioxidant with Antitumour Activity from Serratia ureilytica Using Squid Pen as Fermentation Substrate [Elektronische Daten] [Yao-Haur Kuo, Tzu-Wen Liang, Kao-Cheng Liu, Ya-Wen Hsu, Hsiu-Ching Hsu, San-Lang Wang] The antioxidant activity of the culture supernatant of Serratia ureilytica TKU013 with squid pen as the sole carbon/nitrogen source was assessed by three methods, and the phenolic contents were assayed. The supernatant with the highest antioxidant activity was further purified by liquid-liquid partition, revealing the ethyl acetate extract exhibited the strongest antioxidant activity and the highest total phenolic content. Eight fractions were retrieved from silica gel column chromatography of this extract, designated F1-F8. F4 was found to possess the strong antioxidative activity and the highest total phenolic content and also exhibited strong cytotoxic activities against two different tumoural cell lines. A new compound (Serranticin) with antioxidant and antitumor activity was obtained from F4. The structure of Serranticin is analogous to that of siderophores (hexacoordinated catecholamine), which are iron chelators. As such, Serranticin has the potential for use as a deferration agent in various iron overload diseases. Springer Science+Business Media, LLC, 2010 Serratia ureilytica nationallicence Squid pen wastes nationallicence Antioxidant activity nationallicence Antitumoural activity nationallicence Serranticin nationallicence Kuo Yao-Haur Division of Herbal Medicines and Natural Products, National Research Institute of Chinese Medicine, 112, Taipei, Taiwan aut Liang Tzu-Wen Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut Liu Kao-Cheng Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut Hsu Ya-Wen Division of Herbal Medicines and Natural Products, National Research Institute of Chinese Medicine, 112, Taipei, Taiwan aut Hsu Hsiu-Ching Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut Wang San-Lang Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 451-461 1436-2228 13:3<451 2011 13 10126 https://doi.org/10.1007/s10126-010-9316-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9316-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuo Yao-Haur Division of Herbal Medicines and Natural Products, National Research Institute of Chinese Medicine, 112, Taipei, Taiwan aut NATIONALLICENCE 700 1- Liang Tzu-Wen Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut NATIONALLICENCE 700 1- Liu Kao-Cheng Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut NATIONALLICENCE 700 1- Hsu Ya-Wen Division of Herbal Medicines and Natural Products, National Research Institute of Chinese Medicine, 112, Taipei, Taiwan aut NATIONALLICENCE 700 1- Hsu Hsiu-Ching Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut NATIONALLICENCE 700 1- Wang San-Lang Department of Chemistry, Tamkang University, 251, Tamsui, Taiwan aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 451-461 1436-2228 13:3<451 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer