caa a22 4500 445318414 CHVBK 20180317142655.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10126-010-9327-6 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9327-6 Mitochondrial DNA Introgression in the European Abalone Haliotis tuberculata tuberculata [Elektronische Daten] Evidence for Experimental mtDNA Paternal Inheritance and a Natural Hybrid Sequence [Alain Van Wormhoudt, Valérie Roussel, Gercende Courtois, Sylvain Huchette] Two subspecies of the European abalone have been morphologically recognized: Haliotis tuberculata tuberculata, present in the North Atlantic, and Haliotis tuberculata coccinea, present in the Canary Islands. Among the different nuclear markers used to differentiate these two subspecies, the sperm lysin gene was the most reliable, leading to a 2.2% divergence. Concerning the subunit I of the mitochondrial cytochrome oxydase gene (COI), we observed a difference of 3.3% between the two subspecies. In the North Atlantic, an introgression of mitochondrial DNA from H. tuberculata coccinea to H. tuberculata tuberculata was evident in around 30% of individuals. Due to this difference, we were able to experimentally detect the transfer of paternal mitochondrial DNA (mtDNA) by specific quantitative polymerase chain reaction measurements. The presence of the two mtDNA signatures was also detected in 20% of individuals tested in the field. Moreover, one mt DNA hybrid sequence was identified. The sequencing of this mitochondrial DNA hybrid revealed a mosaic structure with many specific mutations. The origin of this hybrid sequence is discussed. Springer Science+Business Media, LLC, 2010 Abalone nationallicence Haliotis mtDNA introgression nationallicence mtDNA paternal inheritance nationallicence mtDNA natural hybrid sequence nationallicence Van Wormhoudt Alain CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut Roussel Valérie CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut Courtois Gercende Grupo de Investigación en Acuicultura (ICCM & IUSA), Telde, P.O. Box56, 35200, Las Palmas, Canary Islands, Spain aut Huchette Sylvain France Haliotis, Kerazan, 29880, Plouguerneau, France aut Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 563-574 1436-2228 13:3<563 2011 13 10126 https://doi.org/10.1007/s10126-010-9327-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9327-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Wormhoudt Alain CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut NATIONALLICENCE 700 1- Roussel Valérie CNRS UMR 7208, Station de Biologie Marine du Muséum National d'Histoire Naturelle, 29900, Concarneau, France aut NATIONALLICENCE 700 1- Courtois Gercende Grupo de Investigación en Acuicultura (ICCM & IUSA), Telde, P.O. Box56, 35200, Las Palmas, Canary Islands, Spain aut NATIONALLICENCE 700 1- Huchette Sylvain France Haliotis, Kerazan, 29880, Plouguerneau, France aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/3(2011-06-01), 563-574 1436-2228 13:3<563 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer