caa a22 4500 44531849X CHVBK 20180317142655.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10126-010-9332-9 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9332-9 Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio [Elektronische Daten] [Yan Li, Peng Xu, Zixia Zhao, Jian Wang, Yan Zhang, Xiao-Wen Sun] A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species. Springer Science+Business Media, LLC, 2010 BAC nationallicence Common carp nationallicence Genome nationallicence Comparative mapping nationallicence Synteny nationallicence Li Yan The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut Xu Peng The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut Zhao Zixia The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut Wang Jian The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut Zhang Yan The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut Sun Xiao-Wen The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 706-712 1436-2228 13:4<706 2011 13 10126 https://doi.org/10.1007/s10126-010-9332-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9332-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yan The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut NATIONALLICENCE 700 1- Xu Peng The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut NATIONALLICENCE 700 1- Zhao Zixia The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut NATIONALLICENCE 700 1- Wang Jian The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut NATIONALLICENCE 700 1- Zhang Yan The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut NATIONALLICENCE 700 1- Sun Xiao-Wen The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, 100141, Beijing, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 706-712 1436-2228 13:4<706 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer