caa a22 4500 445318597 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10126-010-9333-8 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9333-8 Recovery and Phylogenetic Diversity of Culturable Fungi Associated with Marine Sponges Clathrina luteoculcitella and Holoxea sp. in the South China Sea [Elektronische Daten] [Bo Ding, Ying Yin, Fengli Zhang, Zhiyong Li] Sponge-associated fungi represent an important source of marine natural products, but little is known about the fungal diversity and the relationship of sponge-fungal association, especially no research on the fungal diversity in the South China Sea sponge has been reported. In this study, a total of 111 cultivable fungi strains were isolated from two South China Sea sponges Clathrina luteoculcitella and Holoxea sp. using eight different media. Thirty-two independent representatives were selected for analysis of phylogenetic diversity according to ARDRA and morphological characteristics. The culturable fungal communities consisted of at least 17 genera within ten taxonomic orders of two phyla (nine orders of the phylum Ascomycota and one order of the phylum Basidiomycota) including some potential novel marine fungi. Particularly, eight genera of Apiospora, Botryosphaeria, Davidiella, Didymocrea, Lentomitella, Marasmius, Pestalotiopsis, and Rhizomucor were isolated from sponge for the first time. Sponge C. luteoculcitella has greater culturable fungal diversity than sponge Holoxea sp. Five genera of Aspergillus, Davidiella, Fusarium, Paecilomyces, and Penicillium were isolated from both sponges, while 12 genera of Apiospora, Botryosphaeria, Candida, Marasmius, Cladosporium, Didymocrea, Hypocrea, Lentomitella, Nigrospora, Pestalotiopsis, Rhizomucor, and Scopulariopsis were isolated from sponge C. luteoculcitella only. Order Eurotiales especially genera Penicillium, Aspergillus, and order Hypocreales represented the dominant culturable fungi in these two South China Sea sponges. Nigrospora oryzae strain PF18 isolated from sponge C. luteoculcitella showed a strong and broad spectrum antimicrobial activities suggesting the potential for antimicrobial compounds production. Springer Science+Business Media, LLC, 2010 Marine sponge nationallicence Clathrina luteoculcitella nationallicence Holoxea sp nationallicence Fungus nationallicence Phylogenetic diversity nationallicence Antimicrobial activity nationallicence Ding Bo Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, 20240, Shanghai, People's Republic of China aut Yin Ying Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, 20240, Shanghai, People's Republic of China aut Zhang Fengli Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, 20240, Shanghai, People's Republic of China aut Li Zhiyong School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China aut Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 713-721 1436-2228 13:4<713 2011 13 10126 https://doi.org/10.1007/s10126-010-9333-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9333-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ding Bo Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, 20240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yin Ying Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, 20240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Fengli Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, 20240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Zhiyong School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 713-721 1436-2228 13:4<713 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer