caa a22 4500 445318600 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10126-010-9330-y doi (NATIONALLICENCE)springer-10.1007/s10126-010-9330-y Implications of the Up-regulation of Genes Encoding Protein Degradation Enzymes and Heat Shock Protein 90 for Intertidal Green Macroalga Ulva fasciata Against Hypersalinity-Induced Protein Oxidation [Elektronische Daten] [Ming-Shiuan Sung, Yuan-Ting Hsu, Kuan-Lin Ho, Tse-Min Lee] The molecular acclimation of intertidal green macroalga Ulva fasciata Delile to high salinity stress were examined by the construction of a forward cDNA library via the suppressive subtractive hybridization between 30‰ and 90‰ (24h) and by the time course dynamics of several abundantly expressed genes. Among the genes with known sequences, the expressed sequence tags are abundant in the function of protein synthesis (ribosomal protein) and destination. The cDNAs of ATP-dependent Clp protease (UfClpC), 20S proteasome β-subunit type 1 domain (UfPbf1), ubiquitin-conjugating enzyme E2 I (UfUbc9), and heat shock protein 90A (UfHsp90A) were cloned. UfClpC transcript increased 3h after 90‰ treatment, followed by a decrease, while UfPbf1 and UfUbc9 transcripts increased after 12h and decreased at 48h. The transcripts of UfHsp90A increased 1h after 90‰ treatment, followed by a drop and to the control level at 48h. Protease activity increased 3h after 90‰ treatment and decreased to the control level at 48h. H2O2 contents increased 1h after 90‰ treatment and then remained unchanged, but protein carbonyl group contents increased after 48h. The treatments of reactive oxygen species scavengers partially alleviated 90‰ damage (partial growth rescue) and suppressed the increases in H2O2 content, protein carbonyl group content, protease activity, and UfClpC, UfPbf1, UfUbc9, and UfHsp90A transcripts by 90‰. The induction of specific chaperones and proteases at the molecular level for protein quality control can be considered as one of the molecular mechanisms of hypersalinity acclimation in U. fasciata. Springer Science+Business Media, LLC, 2010 Clp (caseinolytic protease) nationallicence Heat shock protein 90 nationallicence Proteasome-ubiquitin nationallicence Hypersalinity nationallicence Protein oxidation nationallicence Ulva fasciata nationallicence Sung Ming-Shiuan Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut Hsu Yuan-Ting Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut Ho Kuan-Lin Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut Lee Tse-Min Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 684-694 1436-2228 13:4<684 2011 13 10126 https://doi.org/10.1007/s10126-010-9330-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9330-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sung Ming-Shiuan Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Hsu Yuan-Ting Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Ho Kuan-Lin Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Lee Tse-Min Institute of Marine Biology, National Sun Yat-sen University, 804, Kaohsiung, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 684-694 1436-2228 13:4<684 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer