caa a22 4500 445318619 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10126-010-9343-6 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9343-6 Occurrence of a Silicatein Gene in Glass Sponges (Hexactinellida: Porifera) [Elektronische Daten] [Galina Veremeichik, Yuri Shkryl, Victor Bulgakov, Sergey Shedko, Valery Kozhemyako, Svetlana Kovalchuk, Vladimir Krasokhin, Yuri Zhuravlev, Yuri Kulchin] Silicatein genes are involved in spicule formation in demosponges (Demospongiae: Porifera). However, numerous attempts to isolate silicatein genes from glass sponges (Hexactinellida: Porifera) resulted in a limited success. In the present investigation, we performed analysis of potential silicatein/cathepsin transcripts in three different species of glass sponges (Pheronema raphanus, Aulosaccus schulzei, and Bathydorus levis). In total, 472 clones of such transcripts have been analyzed. Most of them represent cathepsin transcripts and only three clones have been found to represent transcripts, which can be related to silicateins. Silicatein transcripts were identified in A. schulzei (Hexactinellida; Lyssacinosida; Rosselidae), and the corresponding gene was called AuSil-Hexa. Expression of AuSil-Hexa in A. schulzei was confirmed by real-time PCR. Comparative sequence analysis indicates high sequence identity of the A. schulzei silicatein with demosponge silicateins described previously. A phylogenetic analysis indicates that the AuSil-Hexa protein belongs to silicateins. However, the AuSil-Hexa protein contains a catalytic cysteine instead of the conventional serine. Springer Science+Business Media, LLC, 2010 Silicatein nationallicence Cathepsin nationallicence Sponge nationallicence Gene expression nationallicence Biosilification nationallicence Hexactinellida nationallicence Veremeichik Galina Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Shkryl Yuri Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Bulgakov Victor Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Shedko Sergey Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Kozhemyako Valery Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Kovalchuk Svetlana Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Krasokhin Vladimir Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Zhuravlev Yuri Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut Kulchin Yuri Institute for Automation and Control Processes, Far East Branch of Russian Academy of Science, Vladivostok, Russia aut Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 810-819 1436-2228 13:4<810 2011 13 10126 https://doi.org/10.1007/s10126-010-9343-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9343-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Veremeichik Galina Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Shkryl Yuri Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Bulgakov Victor Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Shedko Sergey Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Kozhemyako Valery Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Kovalchuk Svetlana Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Krasokhin Vladimir Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Zhuravlev Yuri Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia aut NATIONALLICENCE 700 1- Kulchin Yuri Institute for Automation and Control Processes, Far East Branch of Russian Academy of Science, Vladivostok, Russia aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 810-819 1436-2228 13:4<810 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer