caa a22 4500 445318627 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10126-010-9334-7 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9334-7 Gram-Positive Marine Bacteria as a Potential Resource for the Discovery of Quorum Sensing Inhibitors [Elektronische Daten] [Margaret Teasdale, Kellye Donovan, Stephanie Forschner-Dancause, David Rowley] Inhibitors of bacterial quorum sensing have been proposed as potentially novel therapeutics for the treatment of certain bacterial diseases. We recently reported a marine Halobacillus salinus isolate that secretes secondary metabolites capable of quenching quorum sensing phenotypes in several Gram-negative reporter strains. To investigate how widespread the production of such compounds may be in the marine bacterial environment, 332 Gram-positive isolates from diverse habitats were tested for their ability to interfere with Vibrio harveyi bioluminescence, a cell signaling-regulated phenotype. Rapid assay methods were employed where environmental isolates were propagated alongside the reporter strain. "Actives” were defined as bacteria that interfered with bioluminescence without visible cell-killing effects (antibiotic activity). A total of 49 bacterial isolates interfered with bioluminescence production in the assays. Metabolite extracts were generated from cultures of the active isolates, and 28 reproduced the bioluminescence inhibition against V. harveyi. Of those 28, five extracts additionally inhibited violacein production by Chromobacterium violaceum. Chemical investigations revealed that phenethylamides and a cyclic dipeptide are two types of secondary metabolites responsible for the observed activities. The active bacterial isolates belonged primarily to either the genus Bacillus or Halobacillus. The results suggest that Gram-positive marine bacteria are worthy of further investigation for the discovery of quorum sensing antagonists. Springer Science+Business Media, LLC, 2010 Quorum sensing nationallicence Drug discovery nationallicence Marine bacteria nationallicence Secondary metabolites nationallicence Teasdale Margaret Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut Donovan Kellye Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut Forschner-Dancause Stephanie Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut Rowley David Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 722-732 1436-2228 13:4<722 2011 13 10126 https://doi.org/10.1007/s10126-010-9334-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9334-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Teasdale Margaret Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 700 1- Donovan Kellye Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 700 1- Forschner-Dancause Stephanie Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 700 1- Rowley David Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 722-732 1436-2228 13:4<722 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer