caa a22 4500 445318635 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10126-010-9340-9 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9340-9 Long-Term Cultivation of Primmorphs from Freshwater Baikal Sponges Lubomirskia baikalensis [Elektronische Daten] [Lubov Chernogor, Natalia Denikina, Sergey Belikov, Alexander Ereskovsky] The work was aimed at performing long-term cultivation of primmorphs in vitro from freshwater sponge Lubomirskia baikalensis (Pallas 1776), collected from Lake Baikal, obtaining its long-term primmorph culture in both natural (NBW) and artificial (ABW) Baikal water and at identifying the impact of different environmental factors on formation and growth of primmorphs. The first fine aggregates of L. baikalensis are formed in vitro 10-15min after dissociation of sponge cells. Epithelization of aggregates begins 4h later after the dissociation. Young primmorphs are formed 1 or 2days later. The surface of primmorphs is covered with a layer of exopinacocytes. The primmorphs remain viable for more than 10months at 3-6°C. Over 50% of primmorphs in NBW and 25% in ABW are attached to the substrate and grow like adult sponges. Thus, the long-term primmorph cultivation in vitro allows the creation of a controlled live model system under experimental conditions. The results of this work will allow the creation of a cell culture collection of Baikal freshwater sponges for studying morphogenesis of primmorphs during cultivation at different stages and transdifferentiation of their cells, physiological functions of sponge cells, processes of spiculogenesis, identification of proteins involved in biomineralization process, decoding of their genes, as well as a spectrum of secondary metabolites. Springer Science+Business Media, LLC, 2011 Freshwater sponges nationallicence Lake Baikal nationallicence Lubomirskia baikalensis nationallicence Dissociated cells nationallicence Primmorphs nationallicence Long-term cultivation nationallicence Chernogor Lubov Laboratory of Analytical Bioorganic Chemistry, Limnological Institute of the Siberian Branch of Russian Academy of Sciences, 3 Ulan-Batorskaya St., 664033, Irkutsk, Russia aut Denikina Natalia Laboratory of Analytical Bioorganic Chemistry, Limnological Institute of the Siberian Branch of Russian Academy of Sciences, 3 Ulan-Batorskaya St., 664033, Irkutsk, Russia aut Belikov Sergey Laboratory of Analytical Bioorganic Chemistry, Limnological Institute of the Siberian Branch of Russian Academy of Sciences, 3 Ulan-Batorskaya St., 664033, Irkutsk, Russia aut Ereskovsky Alexander Department of Embryology, Faculty of Biology and Soils, Saint-Petersburg State University, Universitetskaja nab. 7/9, 199034, St. Petersburg, Russia aut Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 782-792 1436-2228 13:4<782 2011 13 10126 https://doi.org/10.1007/s10126-010-9340-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9340-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chernogor Lubov Laboratory of Analytical Bioorganic Chemistry, Limnological Institute of the Siberian Branch of Russian Academy of Sciences, 3 Ulan-Batorskaya St., 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Denikina Natalia Laboratory of Analytical Bioorganic Chemistry, Limnological Institute of the Siberian Branch of Russian Academy of Sciences, 3 Ulan-Batorskaya St., 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Belikov Sergey Laboratory of Analytical Bioorganic Chemistry, Limnological Institute of the Siberian Branch of Russian Academy of Sciences, 3 Ulan-Batorskaya St., 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Ereskovsky Alexander Department of Embryology, Faculty of Biology and Soils, Saint-Petersburg State University, Universitetskaja nab. 7/9, 199034, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 782-792 1436-2228 13:4<782 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer