caa a22 4500 445318678 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10126-010-9331-x doi (NATIONALLICENCE)springer-10.1007/s10126-010-9331-x Endogenous Transposases Affect Differently Sleeping Beauty and Frog Prince Transposons in Fish Cells [Elektronische Daten] [Jose Gallardo-Gálvez, Teresa Méndez, Julia Béjar, M. Alvarez] Fish cells stably expressing exogenous genes have potential applications in the production of fish recombinant proteins, gene-function studies, gene-trapping, and the production of transgenic fish. However, expression of a gene of interest after random integration may be difficult to predict or control. In the past decade, major contributions have been made in vertebrate-gene transfer, by using tools derived from DNA transposons. Among them, the Sleeping Beauty (SB) and Frog Prince (FP) transposons, derived, respectively, from fish and frog genomes, mediate transposition in a large variety of cells, although with different efficiency. This study was aimed at assessing the activities of the SB and the FP transposases in fish cell lines from genetically distant species (CHSE-214, RTG-2, BF-2, EPC, and SAF-1). Their transpositional ability was evaluated by the plasmid-based excision assay, the colony formation assay, and the footprint patterns. The results reveal that while both transposases are active in all cell lines, the transposition rates and the precision of the transposition are overall higher with FP than SB. Our results also indicated a key role of cell-specific host factors in transposition, which was associated with the presence of Tc1-like endogenous transposases; this effect was more accentuated in the two salmonid cell lines transfected with SB. This result agrees with previous studies supporting the use of transposons in heterologous organisms to prevent from genomic instability and from impeding the precise activity of the exogenous transposase. Springer Science+Business Media, LLC, 2010 Transposon nationallicence Sleeping Beauty nationallicence Frog Prince nationallicence CHSE-214 nationallicence EPC nationallicence BF-2 nationallicence SAF-1 nationallicence RTG-2 nationallicence Gallardo-Gálvez Jose Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut Méndez Teresa Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut Béjar Julia Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut Alvarez M. Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 695-705 1436-2228 13:4<695 2011 13 10126 https://doi.org/10.1007/s10126-010-9331-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9331-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gallardo-Gálvez Jose Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut NATIONALLICENCE 700 1- Méndez Teresa Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut NATIONALLICENCE 700 1- Béjar Julia Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut NATIONALLICENCE 700 1- Alvarez M. Department of Genetics, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/4(2011-08-01), 695-705 1436-2228 13:4<695 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer