caa a22 4500 445318724 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-010-9345-4 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9345-4 Diversity of Coding Sequences and Gene Structures of the Antifungal Peptide Mytimycin (MytM) from the Mediterranean Mussel, Mytilus galloprovincialis [Elektronische Daten] [Molruedee Sonthi, Mylène Toubiana, Alberto Pallavicini, Paola Venier, Philippe Roch] Knowledge on antifungal biomolecules is limited compared to antibacterial peptides. A strictly antifungal peptide from the blue mussel, Mytilus edulis named mytimycin (MytM) was reported in 1996 as partial NH2 33 amino acid sequence. Using back-translations of the previous sequence, MytM-related nucleotide sequences were identified from a normalized Mytilus galloprovincialis expressed sequence tag library. Primers designed from a consensus sequence have been used to obtain a fragment of 560 nucleotides, including the complete coding sequence of 456 nucleotides. Precursor is constituted by a signal peptide of 23 amino acids, followed by MytM of 54 amino acids (6.2-6.3kDa, 12 cysteines) and C-terminal extension of 75 amino acids. Only two major amino acid precursor sequences emerged, one shared by M. galloprovincialis from Venice and Vigo, the other belonging to M. galloprovincialis from Palavas, with nine amino acid differences between the two MytM. Predicted disulfide bonds suggested the presence of two constrained domains joined by amino acidic NIFG track. Intriguing was the presence of conserved canonical EF hand-motif located in the C-terminus extension of the precursor. The MytM gene was found interrupted by two introns. Intron 2 existed in two forms, a long (1,112 nucleotides) and a short (716 nucleotides) one resulting from the removal of the central part of the long one. Both the short (GenBank FJ804479) and the long (GenBank FJ804478) genes are simultaneously present in the mussel genome. Springer Science+Business Media, LLC, 2011 Antimicrobial peptide nationallicence Mytimycin nationallicence Polymorphism nationallicence Molecular diversity nationallicence Gene structure nationallicence Innate immunity nationallicence Bivalve nationallicence Mussels nationallicence Mollusks nationallicence Mytilus nationallicence EST : Expressed sequence tag nationallicence gDNA : Genomic DNA nationallicence MytM : Mature moiety of mytimycin precursor nationallicence MytM-P : Mature moiety of mytimycin from Palavas nationallicence MytM-V : Mature moiety of mytimycin from Vigo and Venice nationallicence UTR : Untranslated region nationallicence Sonthi Molruedee Ecosystèmes Lagunaires, CNRS-Université Montpellier 2, cc093, place E. Bataillon, 34095, Montpellier, Cedex 05, France aut Toubiana Mylène Ecosystèmes Lagunaires, CNRS-Université Montpellier 2, cc093, place E. Bataillon, 34095, Montpellier, Cedex 05, France aut Pallavicini Alberto Department of Life Science, University of Trieste, P.le Valmaura, 9, 34148, Trieste, Italy aut Venier Paola Department of Biology, University of Padova, Via Bassi 58/B, 35131, Padova, Italy aut Roch Philippe Ecosystèmes Lagunaires, CNRS-Université Montpellier 2, cc093, place E. Bataillon, 34095, Montpellier, Cedex 05, France aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 857-867 1436-2228 13:5<857 2011 13 10126 https://doi.org/10.1007/s10126-010-9345-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9345-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sonthi Molruedee Ecosystèmes Lagunaires, CNRS-Université Montpellier 2, cc093, place E. Bataillon, 34095, Montpellier, Cedex 05, France aut NATIONALLICENCE 700 1- Toubiana Mylène Ecosystèmes Lagunaires, CNRS-Université Montpellier 2, cc093, place E. Bataillon, 34095, Montpellier, Cedex 05, France aut NATIONALLICENCE 700 1- Pallavicini Alberto Department of Life Science, University of Trieste, P.le Valmaura, 9, 34148, Trieste, Italy aut NATIONALLICENCE 700 1- Venier Paola Department of Biology, University of Padova, Via Bassi 58/B, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Roch Philippe Ecosystèmes Lagunaires, CNRS-Université Montpellier 2, cc093, place E. Bataillon, 34095, Montpellier, Cedex 05, France aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 857-867 1436-2228 13:5<857 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer