caa a22 4500 445318732 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-010-9360-5 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9360-5 Production of Cold-Adapted Amylase by Marine Bacterium Wangia sp. C52: Optimization, Modeling, and Partial Characterization [Elektronische Daten] [Jianguo Liu, Zhiqiang Zhang, Zhiqiang Liu, Hu Zhu, Hongyue Dang, Jianren Lu, Zhanfeng Cui] The aim of this work was to optimize the fermentation parameters in the shake-flask culture of marine bacterium Wangia sp. C52 to increase cold-adapted amylase production using two statistical experimental methods including Plackett-Burman design, which was applied to find the key ingredients for the best medium composition, and response surface methodology, which was used to determine the optimal concentrations of these components. The results showed starch, tryptone, and initial pH had significant effects on the cold-adapted amylase production. A central composite design was then employed to further optimize these three factors. The experimental results indicated that the optimized composition of medium was 6.38g L−1 starch, 33.84g L−1 tryptone, 3.00g L−1 yeast extract, 30g L−1 NaCl, 0.60g L−1 MgSO4 and 0.56g L−1 CaCl2. The optimized cultivation conditions for amylase production were pH7.18, a temperature of 20°C, and a shaking speed of 180rpm. Under the proposed optimized conditions, the amylase experimental yield (676.63U mL−1) closely matched the yield (685.60U mL−1) predicted by the statistical model. The optimization of the medium contributed to tenfold higher amylase production than that of the control in shake-flask experiments. Springer Science+Business Media, LLC, 2011 Cold-adapted amylase nationallicence Optimization nationallicence Plackett-Burman design nationallicence Response surface methodology nationallicence Wangia sp nationallicence Liu Jianguo Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Zhang Zhiqiang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Liu Zhiqiang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Zhu Hu Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Dang Hongyue Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Lu Jianren Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Cui Zhanfeng Department of Engineering Science, University of Oxford, OX1 3PJ, Oxford, UK aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 837-844 1436-2228 13:5<837 2011 13 10126 https://doi.org/10.1007/s10126-010-9360-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9360-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Jianguo Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zhiqiang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Liu Zhiqiang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Hu Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Dang Hongyue Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Lu Jianren Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Cui Zhanfeng Department of Engineering Science, University of Oxford, OX1 3PJ, Oxford, UK aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 837-844 1436-2228 13:5<837 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer