caa a22 4500 445318740 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-010-9351-6 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9351-6 Metagenomic Analysis of 0.2-μm-Passable Microorganisms in Deep-Sea Hydrothermal Fluid [Elektronische Daten] [Ryosuke Nakai, Takashi Abe, Haruko Takeyama, Takeshi Naganuma] We pyrosequenced the bulk DNA extracted from microorganisms that passed through 0.2-μm-pore-size filters and trapped by 0.1-μm-pore-size filters in the hydrothermal fluid of the Mariana Trough. Using the 454-FLX sequencer, we generated 202,648 sequences with an average length of 173.8 bases. Functional profiles were assigned by the SEED Annotation Engine. In the metagenome of the 0.2-μm-passable microorganisms, genes related to membrane function, including potassium homeostasis classified as membrane transport, and multidrug-resistance efflux pumps classified as virulence, were dominant. There was a higher proportion of genes pertinent to the subsystem of membrane transport in our metagenomic library than in other oceanic and hydrothermal vent metagenomes. Genes associated with a RND-type efflux transporter for exogenous substances were specifically identified in the present study. After a comparative analysis with the genome of the known ultramicrobacterium Sphingopyxis alaskensis RB2256, we discovered 1,542 cases of significant hits (E < 1 × 10−2) in our metagenome, and 1,172 of those were related to the DNA repair protein RadA. In this way, the microbial functional profile of 0.2-μm-passable fraction in the present study differs from oceanic metagenomes in the 0.2-μm-trapped fractions and hydrothermal vent metagenomes reported in previous research. Springer Science+Business Media, LLC, 2011 Metagenomics nationallicence Deep sea nationallicence Environmental DNA nationallicence Comparative genomics nationallicence Ultra-micro-sized organisms nationallicence Nakai Ryosuke Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, 739-8528, Higashi-Hiroshima, Hiroshima, Japan aut Abe Takashi Nagahama Institute of Bio-Science and Technology, 1266, Tamura-cho, 526-0829, Nagahama, Shiga, Japan aut Takeyama Haruko Department of Life Science and Medical Bio-Science, Waseda University, 2-2 Wakamatsu-cho, 162-8480, Shinjuku, Tokyo, Japan aut Naganuma Takeshi Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, 739-8528, Higashi-Hiroshima, Hiroshima, Japan aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 900-908 1436-2228 13:5<900 2011 13 10126 https://doi.org/10.1007/s10126-010-9351-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9351-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakai Ryosuke Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, 739-8528, Higashi-Hiroshima, Hiroshima, Japan aut NATIONALLICENCE 700 1- Abe Takashi Nagahama Institute of Bio-Science and Technology, 1266, Tamura-cho, 526-0829, Nagahama, Shiga, Japan aut NATIONALLICENCE 700 1- Takeyama Haruko Department of Life Science and Medical Bio-Science, Waseda University, 2-2 Wakamatsu-cho, 162-8480, Shinjuku, Tokyo, Japan aut NATIONALLICENCE 700 1- Naganuma Takeshi Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, 739-8528, Higashi-Hiroshima, Hiroshima, Japan aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 900-908 1436-2228 13:5<900 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer