caa a22 4500 445318767 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-011-9366-7 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9366-7 Genetic Sex Identification and the Potential Evolution of Sex Determination in Pacific Halibut ( Hippoglossus stenolepis ) [Elektronische Daten] [Heather Galindo, Timothy Loher, Lorenz Hauser] The discovery of genetic markers linked to physiological traits in wild populations is increasingly desired for ecological and evolutionary studies, as well as to inform management decisions. However, identifying such markers often requires a large investment of both time and money. Serendipitously, in a recent microsatellite survey, we discovered three out of 16 microsatellite loci that were correlated to the female sex in Pacific halibut (Hippoglossus stenolepis). These three loci were screened in 550 Pacific halibut to determine their accuracy at identifying sex. Genetic assignment successfully identified sex in 92% of individuals from sample collections spanning 3,000km and 9years. All but two of 287 females had one copy of a characteristic allele for at least one of the three microsatellite loci, resulting in consistent heterozygote excess in females. This pattern is consistent with the hypothesis that females are the heterogametic sex in Pacific halibut, which thus may have a different sex-determination pattern than the closely related Atlantic halibut (Hippoglossus hippoglossus). A rapid divergence of sex-determining mechanisms could be either a cause or consequence of speciation between Pacific and Atlantic halibut. In either case, the ability to genetically identify sex in individual Pacific halibut provides a new tool for ecological studies, fisheries management, and insight into the evolution of sex determination in flatfish. Springer Science+Business Media, LLC, 2011 Pacific halibut nationallicence Sex identification nationallicence Sex determination nationallicence Genetics nationallicence Marine fish nationallicence Galindo Heather School of Aquatic and Fishery Sciences, University of Washington, Box355020, 98195-5020, Seattle, WA, USA aut Loher Timothy International Pacific Halibut Commission, 2320 Commodore Way, Suite 300, 98199, Seattle, WA, USA aut Hauser Lorenz School of Aquatic and Fishery Sciences, University of Washington, Box355020, 98195-5020, Seattle, WA, USA aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1027-1037 1436-2228 13:5<1027 2011 13 10126 https://doi.org/10.1007/s10126-011-9366-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9366-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Galindo Heather School of Aquatic and Fishery Sciences, University of Washington, Box355020, 98195-5020, Seattle, WA, USA aut NATIONALLICENCE 700 1- Loher Timothy International Pacific Halibut Commission, 2320 Commodore Way, Suite 300, 98199, Seattle, WA, USA aut NATIONALLICENCE 700 1- Hauser Lorenz School of Aquatic and Fishery Sciences, University of Washington, Box355020, 98195-5020, Seattle, WA, USA aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1027-1037 1436-2228 13:5<1027 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer