caa a22 4500 445318783 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-011-9364-9 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9364-9 Characterization of a Non-fibrillar-Related Collagen in the Mollusc Haliotis tuberculata and its Biological Activity on Human Dermal Fibroblasts [Elektronische Daten] [Christophe Fleury, Antoine Serpentini, Magdalini Kypriotou, Emmanuelle Renard, Philippe Galéra, Jean-Marc Lebel] In invertebrates, members of the collagen family have been found in various phyla. Surprisingly, in mollusc, little is known about such molecules. In this study, we characterize the full-length abalone type IV collagen and we analysed its biological effects on human fibroblast in order to gain insights about this molecule in molluscs and particularly clues about its roles. We screened a cDNA library of Haliotis tuberculata hemocytes. The expression pattern of the transcript is determined using real-time polymerase chain reaction and in situ hybridization. The close identity between α1(IV) C-terminal domain and the vertebrate homologue led us to produce, purify and test in vitro a recombinant protein corresponding to this region using human dermal fibroblasts cell culture. The biological effects were evaluated on proliferation and on differentiation. We found that the 5,334-bp open reading frame transcript encodes a protein of 1,777 amino acids, including an interrupted 1,502-residue collagenous domain and a 232-residue C-terminal non-collagenous domain. The expression pattern of this transcript is mainly found in the mantle and hemocytes. The recombinant protein corresponding α1(IV) C-terminal domain increased fibroblast proliferation by 69% and doubled collagen synthesis produced in primary cultures. This work provides the first complete primary structure of a mollusc non-fibrillar collagen chain and the biological effects of its C-terminal domain on human cells. In this study, we prove that the NC1 domain from a molluscan collagen can improve human fibroblast proliferation as well as differentiation. Springer Science+Business Media, LLC, 2011 Collagen nationallicence Mollusc nationallicence Hemocytes nationallicence Matrikine nationallicence Fibroblasts nationallicence BM : Basement membranes nationallicence ECM : Extracellular matrix nationallicence NC1 : Non-collagenous domain 1 nationallicence NC1 FL : Non-collagenous domain 1 full length nationallicence Fleury Christophe UMR 100 IFREMER "Physiologie et Ecophysiologie des Mollusques Marins”—IFR 146 ICORE—IBFA—Esplanade de la Paix, Université de Caen Basse-normandie, 14032, Caen, France aut Serpentini Antoine UMR 100 IFREMER "Physiologie et Ecophysiologie des Mollusques Marins”—IFR 146 ICORE—IBFA—Esplanade de la Paix, Université de Caen Basse-normandie, 14032, Caen, France aut Kypriotou Magdalini Laboratoire Matrice Extracellulaire et Pathologie, EA 3214, IFR 146 ICORE, Faculté de Médecine, Université de Caen Basse-Normandie, CHU niveau 3, avenue de la Côte de nacre, 14032, Caen, France aut Renard Emmanuelle Laboratoire Matrice Extracellulaire et Pathologie, EA 3214, IFR 146 ICORE, Faculté de Médecine, Université de Caen Basse-Normandie, CHU niveau 3, avenue de la Côte de nacre, 14032, Caen, France aut Galéra Philippe Laboratoire Matrice Extracellulaire et Pathologie, EA 3214, IFR 146 ICORE, Faculté de Médecine, Université de Caen Basse-Normandie, CHU niveau 3, avenue de la Côte de nacre, 14032, Caen, France aut Lebel Jean-Marc UMR 100 IFREMER "Physiologie et Ecophysiologie des Mollusques Marins”—IFR 146 ICORE—IBFA—Esplanade de la Paix, Université de Caen Basse-normandie, 14032, Caen, France aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1003-1016 1436-2228 13:5<1003 2011 13 10126 https://doi.org/10.1007/s10126-011-9364-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9364-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fleury Christophe UMR 100 IFREMER "Physiologie et Ecophysiologie des Mollusques Marins”—IFR 146 ICORE—IBFA—Esplanade de la Paix, Université de Caen Basse-normandie, 14032, Caen, France aut NATIONALLICENCE 700 1- Serpentini Antoine UMR 100 IFREMER "Physiologie et Ecophysiologie des Mollusques Marins”—IFR 146 ICORE—IBFA—Esplanade de la Paix, Université de Caen Basse-normandie, 14032, Caen, France aut NATIONALLICENCE 700 1- Kypriotou Magdalini Laboratoire Matrice Extracellulaire et Pathologie, EA 3214, IFR 146 ICORE, Faculté de Médecine, Université de Caen Basse-Normandie, CHU niveau 3, avenue de la Côte de nacre, 14032, Caen, France aut NATIONALLICENCE 700 1- Renard Emmanuelle Laboratoire Matrice Extracellulaire et Pathologie, EA 3214, IFR 146 ICORE, Faculté de Médecine, Université de Caen Basse-Normandie, CHU niveau 3, avenue de la Côte de nacre, 14032, Caen, France aut NATIONALLICENCE 700 1- Galéra Philippe Laboratoire Matrice Extracellulaire et Pathologie, EA 3214, IFR 146 ICORE, Faculté de Médecine, Université de Caen Basse-Normandie, CHU niveau 3, avenue de la Côte de nacre, 14032, Caen, France aut NATIONALLICENCE 700 1- Lebel Jean-Marc UMR 100 IFREMER "Physiologie et Ecophysiologie des Mollusques Marins”—IFR 146 ICORE—IBFA—Esplanade de la Paix, Université de Caen Basse-normandie, 14032, Caen, France aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1003-1016 1436-2228 13:5<1003 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer