caa a22 4500 445318791 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-011-9362-y doi (NATIONALLICENCE)springer-10.1007/s10126-011-9362-y A New Method to Extract Matrix Proteins Directly from the Secretion of the Mollusk Mantle and the Role of These Proteins in Shell Biomineralization [Elektronische Daten] [Xiaojun Liu, Chang Liu, Lei Chen, Juan Sun, Yujuan Zhou, Qi Li, Guilan Zheng, Guiyou Zhang, Hongzhong Wang, Liping Xie, Rongqing Zhang] Considering the continuous and substantive secretory ability of the mantle in vitro, we report a new technique to produce shell-matrix proteins by inducing the mantle, after removal from the organism's body, to secrete soluble-matrix proteins into phosphate buffer. By this method, a large amount of matrix proteins could be obtained in 2h. Experiments involving in vitro calcium carbonate crystallization and organic framework calcium carbonate crystallization indicated that these proteins retain high bioactivity and play key roles in shell biomineralization. Phosphate buffer-soluble proteins secreted by the margin of the mantles (MSPs) were used to reconstruct the stages in the growth of the prismatic layer of the decalcified organic frameworks. The MSPs were observed to aggregate calcites in vitro, and this ability enabled the mollusk to form big calcites in the prismatic layer. During shell biomineralization, an important stage after the self-assembly of the biomacromolecules and the formation of crystals is the assembly of the two parts to form a firm structure. Moreover, a new type of matrix protein, functioning as the binding factor between the crystals and the organic frameworks, was shown to exist in the phosphate buffer-soluble proteins secreted by the central part of mantles (CSPs). Nanoscale-sized bowl-like aragonites, with heights of ∼800nm, were induced by CSPs in vitro. This method is a successful example of obtaining functional proteins through secretion by animal tissues. Springer Science+Business Media, LLC, 2011 Mollusks nationallicence Pearl oyster nationallicence Mantle secretion nationallicence Shell-matrix proteins nationallicence Biomineralization nationallicence Liu Xiaojun Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Liu Chang Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Chen Lei Department of Gynecology and Obstetrics, The Naval General Hospital, 100037, Beijing, China aut Sun Juan Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Zhou Yujuan Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Li Qi Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Zheng Guilan Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Zhang Guiyou Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Wang Hongzhong Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Xie Liping Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Zhang Rongqing Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 981-991 1436-2228 13:5<981 2011 13 10126 https://doi.org/10.1007/s10126-011-9362-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9362-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Xiaojun Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Liu Chang Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Chen Lei Department of Gynecology and Obstetrics, The Naval General Hospital, 100037, Beijing, China aut NATIONALLICENCE 700 1- Sun Juan Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhou Yujuan Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Li Qi Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zheng Guilan Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhang Guiyou Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Wang Hongzhong Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Xie Liping Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhang Rongqing Institute of Marine Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 981-991 1436-2228 13:5<981 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer