caa a22 4500 445318805 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-010-9359-y doi (NATIONALLICENCE)springer-10.1007/s10126-010-9359-y Surface Display of Aeromonas hydrophila GAPDH in Attenuated Vibrio anguillarum to Develop a Noval Multivalent Vector Vaccine [Elektronische Daten] [Yan Zhao, Qin Liu, Xiuhua Wang, Lingyun Zhou, Qiyao Wang, Yuanxing Zhang] Displaying foreign antigens on the surface of attenuated or avirulent bacteria is an important strategy to develop live multivalent vector vaccines. In our previous work, several efficient surface display systems have been established based on outer membrane anchoring elements, which could successfully display heterologous proteins in attenuated Vibrio anguillarum. In this work, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from pathogenic Aeromonas hydrophila LSA34 was fused to seven display systems and introduced into attenuated V. anguillarum strain MVAV6203 (AV) to get seven GAPDH-display strains. The strain AV/pN-gapA showed the best display efficacy of GAPDH and was tested as the multivalent vaccine candidate. Further immune protection evaluation of AV/pN-gapA in turbot (Scophtalmus maximus) demonstrated that the attenuated V. anguillarum with surface-displayed GAPDH of A. hydrophila LSA34 effectively protected turbot from the infections of A. hydrophila and V. anguillarum and showed potential value for further multivalent vaccine development. Springer Science+Business Media, LLC, 2011 Aeromonas hydrophila nationallicence Vibrio anguillarum nationallicence Surface display nationallicence Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nationallicence Multivalent vaccine nationallicence Zhao Yan State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut Liu Qin State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut Wang Xiuhua Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, 166071, Qingdao, China aut Zhou Lingyun State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut Wang Qiyao State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut Zhang Yuanxing State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 963-970 1436-2228 13:5<963 2011 13 10126 https://doi.org/10.1007/s10126-010-9359-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9359-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Yan State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Liu Qin State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Wang Xiuhua Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, 166071, Qingdao, China aut NATIONALLICENCE 700 1- Zhou Lingyun State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Wang Qiyao State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Yuanxing State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 963-970 1436-2228 13:5<963 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer