caa a22 4500 445318813 CHVBK 20180317142656.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-011-9365-8 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9365-8 Induced Gynogenesis in Grass Carp ( Ctenopharyngodon idellus ) Using Irradiated Sperm of Allotetraploid Hybrids [Elektronische Daten] [Hong Zhang, ShaoJun Liu, Chun Zhang, Min Tao, LiangYue Peng, CuiPing You, Jun Xiao, Yi Zhou, GongJian Zhou, KaiKun Luo, Yun Liu] Grass carp (Ctenopharyngodon idellus) eggs were activated by UV-irradiated diploid sperm of allotetraploid hybrids derived from red crucian carp (♀) × common carp (♂) and then duplicated by cold shock in 4-6°C water for 10-12min. Different cold shock initiation times resulted in two types of diploid gynogenetic grass carp: meiotic gynogenetic (meiG) and mitotic gynogenetic (mitG). Over a 5-year period, a total of 17,170 meiG and 1,080 mitG fry were produced and 6,862 meiG and 372 mitG grass carp survived. The gynogenetic fish were confirmed by morphological characteristics, chromosome examination, and microsatellite DNA analysis. The morphological traits of the gynogenetic grass carp were similar to those of wild diploid grass carp. Normal gynogenetic fish were identified as diploid with 48 chromosomes by chromosomal metaphases examination, while nonviable abnormal embryos were detected as haploid with 24 chromosomes. Microsatellite DNA analysis indicated that after one generation of gynogenesis, the genetic purity of meiG and mitG grass carp was significantly increased over that of wild grass carp. In addition, both meiG and mitG grass carp groups were 100% female, and 88% of these showed normal ovary development. Thus, the sex determination mechanism in female grass carp was homogamety. The ability to establish pure all-female groups of meiG and mitG grass carp should be a valuable contribution to both fish genetics and grass carp breeding. Springer Science+Business Media, LLC, 2011 Grass carp nationallicence Gynogenesis nationallicence Microsatellite nationallicence Sex determination nationallicence Zhang Hong Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Liu ShaoJun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Zhang Chun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Tao Min Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Peng LiangYue Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut You CuiPing Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Xiao Jun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Zhou Yi Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Zhou GongJian Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Luo KaiKun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Liu Yun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1017-1026 1436-2228 13:5<1017 2011 13 10126 https://doi.org/10.1007/s10126-011-9365-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9365-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Hong Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Liu ShaoJun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Chun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Tao Min Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Peng LiangYue Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- You CuiPing Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Jun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Yi Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Zhou GongJian Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Luo KaiKun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yun Key Laboratory of Protein Chemistry and Fish Developmental Biology of the Education Ministry of China, College of Life Sciences, Hunan Normal University, 410081, Changsha, People's Republic of China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1017-1026 1436-2228 13:5<1017 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer