caa a22 4500 445318821 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-010-9361-4 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9361-4 A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas [Elektronische Daten] [Charlotte Corporeau, Agnès Groisillier, Alexandra Jeudy, Tristan Barbeyron, Elodie Fleury, Caroline Fabioux, Mirjam Czjzek, Arnaud Huvet] The transforming growth factor (TGF)-β superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-β gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-β appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-β family members. The function of og TGF-β protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-β protein, and we demonstrate that og TGF-β is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-β in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-β function tends to reduce gonadic area. We conclude that mature og TGF-β probably functions as an activator of germ cells development in oyster. Springer Science+Business Media, LLC, 2011 Transforming growth factor-β nationallicence Bacterial expression nationallicence In vivo antibody inhibition nationallicence Reproduction nationallicence Crassostrea gigas nationallicence Marine bivalve nationallicence Corporeau Charlotte UMR 100 Ifremer-centre de Brest, PE2M, 29280, Plouzané, France aut Groisillier Agnès CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut Jeudy Alexandra CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut Barbeyron Tristan CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut Fleury Elodie UMR 100 Ifremer-centre de Brest, PE2M, 29280, Plouzané, France aut Fabioux Caroline UMR CNRS 6539, LEMAR, Université de Bretagne Occidentale, IUEM, 29280, Plouzané, France aut Czjzek Mirjam CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut Huvet Arnaud UMR 100 Ifremer-centre de Brest, PE2M, 29280, Plouzané, France aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 971-980 1436-2228 13:5<971 2011 13 10126 https://doi.org/10.1007/s10126-010-9361-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9361-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Corporeau Charlotte UMR 100 Ifremer-centre de Brest, PE2M, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Groisillier Agnès CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut NATIONALLICENCE 700 1- Jeudy Alexandra CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut NATIONALLICENCE 700 1- Barbeyron Tristan CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut NATIONALLICENCE 700 1- Fleury Elodie UMR 100 Ifremer-centre de Brest, PE2M, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Fabioux Caroline UMR CNRS 6539, LEMAR, Université de Bretagne Occidentale, IUEM, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Czjzek Mirjam CNRS-UPMC Univ Paris 6, UMR 7139 Végétaux marins et Biomolécules, LIA DIAMS, Station Biologique, 29682, Roscoff, France aut NATIONALLICENCE 700 1- Huvet Arnaud UMR 100 Ifremer-centre de Brest, PE2M, 29280, Plouzané, France aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 971-980 1436-2228 13:5<971 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer