caa a22 4500 44531883X CHVBK 20180317142657.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-011-9367-6 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9367-6 Transient Gene Expression System Established in Porphyra yezoensis Is Widely Applicable in Bangiophycean Algae [Elektronische Daten] [Ryo Hirata, Megumu Takahashi, Naotsune Saga, Koji Mikami] The establishment of transient gene expression systems in the marine red macroalga Porphyra yezoensis has been useful for the molecular analysis of cellular processes in this species. However, there has been no successful report about the expression of foreign genes in other red macroalgae, which has impeded the broader understanding of the molecular biology of these species. We therefore examined whether the P. yezoensis transient gene expression system was applicable to other red macroalgae. The results indicated that a codon-optimized GUS, designated PyGUS, and plant-adapted sGFP(S65T) were successfully expressed under the control of the P. yezoensis PyAct1 promoter in gametophytic cells of six Porphyra species and also in Bangia fuscopurpurea, all of which are classified as Bangiophyceae. In contrast, there were no reporter-expressing cells in the Florideophycean algae examined. These results indicate the availability of PyGUS and sGFP as reporters and the 5′ upstream region of the PyAct1 gene as a heterologous promoter for transient gene expression in Bangiophycean algae, which could provide a clue to the efficient expression of foreign genes and transformation in marine red macroalgae. Springer Science+Business Media, LLC, 2011 Bangiophyceae nationallicence Porphyra nationallicence PyAct1 promoter nationallicence PyGUS nationallicence sGFP nationallicence Transient gene expression nationallicence Hirata Ryo Graduate School of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Japan aut Takahashi Megumu Graduate School of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Japan aut Saga Naotsune Faculty of Fisheries Science, Hokkaido University, 041-8611, Hakodate, Japan aut Mikami Koji Faculty of Fisheries Science, Hokkaido University, 041-8611, Hakodate, Japan aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1038-1047 1436-2228 13:5<1038 2011 13 10126 https://doi.org/10.1007/s10126-011-9367-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9367-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hirata Ryo Graduate School of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Japan aut NATIONALLICENCE 700 1- Takahashi Megumu Graduate School of Fisheries Sciences, Hokkaido University, 041-8611, Hakodate, Japan aut NATIONALLICENCE 700 1- Saga Naotsune Faculty of Fisheries Science, Hokkaido University, 041-8611, Hakodate, Japan aut NATIONALLICENCE 700 1- Mikami Koji Faculty of Fisheries Science, Hokkaido University, 041-8611, Hakodate, Japan aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1038-1047 1436-2228 13:5<1038 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer