caa a22 4500 445318880 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-011-9363-x doi (NATIONALLICENCE)springer-10.1007/s10126-011-9363-x A New Coral Carbonic Anhydrase in Stylophora pistillata [Elektronische Daten] [Anthony Bertucci, Sylvie Tambutté, Claudiu Supuran, Denis Allemand, Didier Zoccola] Scleractinian corals are of particular interest due to their ability to establish an intracellular mutualistic symbiosis with phototrophic dinoflagellates and to deposit high rates of calcium carbonate in their skeleton. Carbonic anhydrases have been shown to play a crucial role in both processes. In this study, we report the molecular cloning and characterization of a novel α-CA in the coral Stylophora pistillata. This enzyme shares homologies with the human isoform CA II and is referred to as STPCA-2. STPCA-2 is 35.2kDa and possesses all key amino acids for catalytic activity. With a ratio between catalytic and Michaelis constants (kcat/Km) of 8.3.107M−1 s−1 is considered as highly active. Owing to its intracellular localisation in the oral endoderm and in the aboral tissue, we propose that STPCA-2 is involved in pH regulation and/or inorganic carbon delivery to symbiont and calcification. Springer Science+Business Media, LLC, 2011 Scleractinian corals nationallicence Carbonic anhydrase nationallicence Symbiosis nationallicence Calcification nationallicence Intracelullar pH nationallicence Bertucci Anthony Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut Tambutté Sylvie Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut Supuran Claudiu Dipartimento di Chimica, University of Florence, via della Lastruccia, 3, Rm. 188, Polo Scientifico, 50019, Sesto Fiorentino (Florence), Italy aut Allemand Denis Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut Zoccola Didier Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 992-1002 1436-2228 13:5<992 2011 13 10126 https://doi.org/10.1007/s10126-011-9363-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9363-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bertucci Anthony Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut NATIONALLICENCE 700 1- Tambutté Sylvie Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut NATIONALLICENCE 700 1- Supuran Claudiu Dipartimento di Chimica, University of Florence, via della Lastruccia, 3, Rm. 188, Polo Scientifico, 50019, Sesto Fiorentino (Florence), Italy aut NATIONALLICENCE 700 1- Allemand Denis Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut NATIONALLICENCE 700 1- Zoccola Didier Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 992-1002 1436-2228 13:5<992 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer