caa a22 4500 445318899 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-011-9368-5 doi (NATIONALLICENCE)springer-10.1007/s10126-011-9368-5 Structural Characterization of Extracellular Polysaccharides Produced by the Marine Fungus Epicoccum nigrum JJY-40 and Their Antioxidant Activities [Elektronische Daten] [Hai-Hong Sun, Wen-Jun Mao, Jie-Ying Jiao, Jia-Chao Xu, Hong-Yan Li, Yin Chen, Xiao-Hui Qi, Yan-Li Chen, Jian Xu, Chun-Qi Zhao, Yu-Jiao Hou, Yu-Pin Yang] Two extracellular polysaccharides, ENP1 and ENP2, were isolated from the fermentation liquid of the marine fungus Epicoccum nigrum JJY-40 by anion-exchange chromatography and gel-filtration chromatography, and their structures were investigated using chemical and spectroscopic methods including methylation analysis and NMR spectroscopy. The results demonstrated that ENP1 was composed of mannose, glucose, and galactose in the molar ratio of 5.0:2.1:1.0, and the main chain of the polysaccharide consisted of (1 → 2)-linked mannose, (1 → 3)-linked mannose, terminal mannose, (1 → 6)-linked glucose, (1 → 4)-linked glucose, and (1 → 4)-linked galactose. ENP2 was composed of mannose, galactose, glucose, and glucuronic acid in a molar ratio of 12.4:11.2:8.3:1.0, and its glycosidic linkage patterns included terminal mannose, (1 → 6)-linked glucose, (1 → 4)-linked galactose, and (1 → 3)-linked mannose. The two polysaccharides had a partially branched structure with branch point located at C-3 position of (1 → 6)-linked glucose residue. The molecular weights of ENP1 and ENP2 were 19.2kDa and 32.7kDa, respectively. Antioxidant properties of the two polysaccharides were evaluated with hydroxyl, superoxide, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and lipid peroxidation inhibition in vitro, and results showed that ENP2 and ENP1 had good antioxidant activities, especially ENP2. ENP2 could be effective as a potential antioxidant. Springer Science+Business Media, LLC, 2011 Marine fungus nationallicence Epicoccum nigrum JJY-40 nationallicence Extracellular polysaccharide nationallicence Structural characterization nationallicence Antioxidant activity nationallicence Sun Hai-Hong Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Mao Wen-Jun Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Jiao Jie-Ying Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Xu Jia-Chao College of Food Science and Technology, Ocean University of China, 266003, Qingdao, China aut Li Hong-Yan Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Chen Yin Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Qi Xiao-Hui Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Chen Yan-Li Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Xu Jian Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Zhao Chun-Qi Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Hou Yu-Jiao Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Yang Yu-Pin Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1048-1055 1436-2228 13:5<1048 2011 13 10126 https://doi.org/10.1007/s10126-011-9368-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-011-9368-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Hai-Hong Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Mao Wen-Jun Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Jiao Jie-Ying Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Xu Jia-Chao College of Food Science and Technology, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Li Hong-Yan Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Chen Yin Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Qi Xiao-Hui Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Chen Yan-Li Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Xu Jian Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Zhao Chun-Qi Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Hou Yu-Jiao Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Yang Yu-Pin Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 1048-1055 1436-2228 13:5<1048 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer