caa a22 4500 445318902 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10126-010-9355-2 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9355-2 Photoautotrophic Production of Lipids by Some Chlorella Strains [Elektronische Daten] [Sarote Sirisansaneeyakul, Somruethai Singhasuwan, Wanna Choorit, Natapas Phoopat, Jose Garcia, Yusuf Chisti] The microalgae Chlorella protothecoides UTEX 25, Chlorella sp. TISTR 8991, and Chlorella sp. TISTR 8990 were compared for use in the production of biomass and lipids under photoautotrophic conditions. Chlorella sp. TISTR 8990 was shown to be potentially suitable for lipid production at 30°C in a culture medium that contained only inorganic salts. For Chlorella sp. TISTR 8990 in optimal conditions in a stirred tank photobioreactor, the lipid productivity was 2.3mgL−1h−1 and after 14days the biomass contained more than 30% lipids by dry weight. To attain this, the nitrogen was provided as KNO3 at an initial concentration of 2.05g L−1 and chelated ferric iron was added at a concentration of 1.2 × 10−5molL−1 on the ninth day. Under the same conditions in culture tubes (36mm outer diameter), the biomass productivity was 2.8-fold greater than in the photobioreactor (0.125m in diameter), but the lipid productivity was only 1.2-fold higher. Thus, the average low-light level in the photobioreactor actually increased the biomass specific lipid production compared to the culture tubes. A light-limited growth model closely agreed with the experimental profiles of biomass production, nitrogen consumption, and lipid production in the photobioreactor. Springer Science+Business Media, LLC, 2011 Microalgae nationallicence Chlorella spp nationallicence Chlorella protothecoides nationallicence Photoautotrophic culture nationallicence Lipid production nationallicence Taguchi method nationallicence Batch production model nationallicence Sirisansaneeyakul Sarote Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 10900, Bangkok, Thailand aut Singhasuwan Somruethai Biotechnology Program, School of Agricultural Technology, Walailak University, 80161, Nakhon Si Thammarat, Thailand aut Choorit Wanna Biotechnology Program, School of Agricultural Technology, Walailak University, 80161, Nakhon Si Thammarat, Thailand aut Phoopat Natapas Institute of Food Research and Product Development, Kasetsart University, 10900, Bangkok, Thailand aut Garcia Jose Department of Chemical Engineering, University of Almería, 04071, Almería, Spain aut Chisti Yusuf School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand aut Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 928-941 1436-2228 13:5<928 2011 13 10126 https://doi.org/10.1007/s10126-010-9355-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9355-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sirisansaneeyakul Sarote Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 10900, Bangkok, Thailand aut NATIONALLICENCE 700 1- Singhasuwan Somruethai Biotechnology Program, School of Agricultural Technology, Walailak University, 80161, Nakhon Si Thammarat, Thailand aut NATIONALLICENCE 700 1- Choorit Wanna Biotechnology Program, School of Agricultural Technology, Walailak University, 80161, Nakhon Si Thammarat, Thailand aut NATIONALLICENCE 700 1- Phoopat Natapas Institute of Food Research and Product Development, Kasetsart University, 10900, Bangkok, Thailand aut NATIONALLICENCE 700 1- Garcia Jose Department of Chemical Engineering, University of Almería, 04071, Almería, Spain aut NATIONALLICENCE 700 1- Chisti Yusuf School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/5(2011-10-01), 928-941 1436-2228 13:5<928 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer