caa a22 4500 445318953 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9276-0 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9276-0 Induction of Ovarian Maturation and Spawning in Penaeus monodon Broodstock by Double-Stranded RNA [Elektronische Daten] [Supattra Treerattrakool, Sakol Panyim, Apinunt Udomkit] Ovarian maturation in crustacean is under the control of gonad-inhibiting hormone (GIH); a neuropeptide secreted from X-organ sinus gland complex in eyestalks. Unilateral eyestalk ablation that partially destroys GIH source is therefore a general practice in Penaeus monodon hatchery to induce ovarian maturation and spawning. Our previous report showed that silencing of GIH expression by GIH-specific double-stranded RNA (GIH-dsRNA) resulted in an increased expression level of vitellogenin in P. monodon, thus suggesting that GIH-dsRNA could be an alternative method to induce ovarian maturation in female P. monodon broodstock. In this study, we further demonstrated that a single injection of GIH-dsRNA into previtellogenic female P. monodon at the concentration of 3µg GIH-dsRNA per gram body weight of shrimp was able to inhibit GIH expression for a minimum of 30days. This dsRNA-mediated GIH silencing led to ovarian maturation and eventual spawning in both domesticated and wild female broodstock, particularly with a comparable effect to eyestalk ablation in wild shrimp. This is the first report that demonstrates a potential strategy to induce ovarian maturation in female P. monodon broodstock by GIH-dsRNA and thus provides a possible substitute for the cruel and detrimental eyestalk ablation practice. Springer Science+Business Media, LLC, 2010 Black tiger shrimp nationallicence Gonad-inhibiting hormone nationallicence Eyestalk ablation nationallicence RNA interference nationallicence Ovarian maturation nationallicence Treerattrakool Supattra Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut Panyim Sakol Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut Udomkit Apinunt Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 163-169 1436-2228 13:2<163 2011 13 10126 https://doi.org/10.1007/s10126-010-9276-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9276-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Treerattrakool Supattra Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut NATIONALLICENCE 700 1- Panyim Sakol Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut NATIONALLICENCE 700 1- Udomkit Apinunt Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 163-169 1436-2228 13:2<163 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer