caa a22 4500 445318961 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9296-9 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9296-9 Development of Expressed Sequence Tags from the Pearl Oyster, Pinctada martensii Dunker [Elektronische Daten] [Aimin Wang, Yan Wang, Zhifeng Gu, Sifa Li, Yaohua Shi, Ximing Guo] The pearl oyster, Pinctada martensii, is the primary species used for the aquaculture production of marine pearls in China and Japan. Genetic tools and resources are needed to study the genome of this species and to understand the molecular basis of development, growth, host defense, pearl formation, and other important traits. In this study, we developed a set of expressed sequence tags (ESTs) for P. martensii. We constructed cDNA libraries from adult tissues and sequenced 7,128 ESTs. Clustering analysis identified 788 contigs (covering 5,769 ESTs) and 1,351 singletons, yielding a total of 2,139 unique genes. Of these unique genes, only 935 had significant (E-value ≤ 0.005) hits in GenBank, and the remaining 1,204 (56.3%) were novel. Most of the known genes are related to cellular structure, protein binding, and metabolic processes. Putative host-defense genes (86) were identified including C-type lectin, ferritin, polyubiquitin, proteases, protease inhibitors, scavenger receptors, heat shock proteins, and RAS oncogenes. The EST sequences developed in this study provide a valuable resource for future efforts on gene identification, marker development, and studies on molecular mechanism of host defense in pearl oysters. Springer Science+Business Media, LLC, 2010 Expressed sequence tags (ESTs) nationallicence Genetic markers nationallicence Host defense genes nationallicence Pearl oyster nationallicence Pinctada martensii nationallicence Aquaculture nationallicence Wang Aimin Key Laboratory of Aquatic Genetic Resources and Aquaculture Ecosystem, Ministry of Agriculture, College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China aut Wang Yan Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, 570228, Haikou, China aut Gu Zhifeng Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, 570228, Haikou, China aut Li Sifa Key Laboratory of Aquatic Genetic Resources and Aquaculture Ecosystem, Ministry of Agriculture, College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China aut Shi Yaohua Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, 570228, Haikou, China aut Guo Ximing Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Avenue, 08349, Port Norris, NJ, USA aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 275-283 1436-2228 13:2<275 2011 13 10126 https://doi.org/10.1007/s10126-010-9296-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9296-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Aimin Key Laboratory of Aquatic Genetic Resources and Aquaculture Ecosystem, Ministry of Agriculture, College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China aut NATIONALLICENCE 700 1- Wang Yan Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, 570228, Haikou, China aut NATIONALLICENCE 700 1- Gu Zhifeng Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, 570228, Haikou, China aut NATIONALLICENCE 700 1- Li Sifa Key Laboratory of Aquatic Genetic Resources and Aquaculture Ecosystem, Ministry of Agriculture, College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China aut NATIONALLICENCE 700 1- Shi Yaohua Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan Key Laboratory of Tropical Hydrobiological Technology, The Ocean College, Hainan University, 570228, Haikou, China aut NATIONALLICENCE 700 1- Guo Ximing Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, 6959 Miller Avenue, 08349, Port Norris, NJ, USA aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 275-283 1436-2228 13:2<275 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer