caa a22 4500 44531897X CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9300-4 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9300-4 Bacterial Community Dynamics in the Marine Sponge Rhopaloeides odorabile Under In Situ and Ex Situ Cultivation [Elektronische Daten] [Nicole Webster, Rose Cobb, Rochelle Soo, Shelley Anthony, Christopher Battershill, Steve Whalan, Elizabeth Evans-Illidge] Cultivation of sponges is being explored to supply biomaterial for the pharmaceutical and cosmetics industries. This study assesses the impact of various cultivation methods on the microbial community within the sponge Rhopaloeides odorabile during: (1) in situ cultivation under natural environmental conditions, (2) ex situ cultivation in small flow-through aquaria and (3) ex situ cultivation in large mesocosm systems. Principal components analysis of denaturing gradient gel electrophoresis profiles indicated a stable microbial community in sponges cultured in situ (grown in the wild) and in sponges cultured ex situ in small flow-through aquaria over 12weeks. In contrast, a shift in the microbial community was detected in sponges cultivated ex situ in large mesocosm aquaria for 12months. This shift included (1) a loss of some stable microbial inhabitants, including members of the Poribacteria, Chloroflexi and Acidobacteria and (2) the addition of new microbes not detected in the wild sponges. Many of these acquired bacteria had highest similarity to known sponge-associated microbes, indicating that the sponge may be capable of actively selecting its microbial community. Alternatively, long-term ex situ cultivation may cause a shift in the dominant microbes that facilitates the growth of the more rare species. The microbial community composition varied between sponges cultivated in mesocosm aquaria with different nutrient concentrations and seawater chemistry, suggesting that these variables play a role in structuring the sponge-associated microbes. The high growth and symbiont stability in R. odorabile cultured in situ confirm that this is the preferred method of aquaculture for this species at this time. Springer Science+Business Media, LLC, 2010 Sponge nationallicence Cultivation nationallicence Marine nationallicence Symbiosis nationallicence Bacteria nationallicence Aquaria nationallicence Webster Nicole Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut Cobb Rose Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut Soo Rochelle Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut Anthony Shelley Great Barrier Reef Marine Park Authority, Townsville, Qld, Australia aut Battershill Christopher Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut Whalan Steve Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut Evans-Illidge Elizabeth Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 296-304 1436-2228 13:2<296 2011 13 10126 https://doi.org/10.1007/s10126-010-9300-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9300-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Webster Nicole Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut NATIONALLICENCE 700 1- Cobb Rose Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut NATIONALLICENCE 700 1- Soo Rochelle Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut NATIONALLICENCE 700 1- Anthony Shelley Great Barrier Reef Marine Park Authority, Townsville, Qld, Australia aut NATIONALLICENCE 700 1- Battershill Christopher Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut NATIONALLICENCE 700 1- Whalan Steve Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut NATIONALLICENCE 700 1- Evans-Illidge Elizabeth Australian Institute of Marine Science, PMB 3, Townsville Mail Centre, 4810, Townsville, Qld, Australia aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 296-304 1436-2228 13:2<296 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer