caa a22 4500 445318996 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9275-1 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9275-1 Identification and Analysis of Muscle-Related Protein Isoforms Expressed in the White Muscle of the Mandarin Fish ( Siniperca chuatsi ) [Elektronische Daten] [Guoqiang Zhang, Wuying Chu, Songnian Hu, Tao Meng, Linlin Pan, Renxue Zhou, Zhen Liu, Jianshe Zhang] To identify muscle-related protein isoforms expressed in the white muscle of the mandarin fish Siniperca chuatsi, we analyzed 5,063 high-quality expressed sequence tags (ESTs) from white muscle cDNA library and predicted the integrity of the clusters annotated to these genes and the physiochemical properties of the putative polypeptides with full length. Up to about 33% of total ESTs were annotated to muscle-related proteins: myosin, actin, tropomyosin/troponin complex, parvalbumin, and Sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCa). Thirty-two isoforms were identified and more than one isoform existed in each of these proteins. Among these isoforms, 14 putative polypeptides were with full length. In addition, about 2% of total ESTs were significantly homologous to "glue” molecules such as alpha-actinins, myosin-binding proteins, myomesin, tropomodulin, cofilin, profilin, twinfilins, coronin-1, and nebulin, which were required for the integrity and maintenance of the muscle sarcomere. The results demonstrated that multiple isoforms of major muscle-related proteins were expressed in S. chuatsi white muscle. The analysis on these isoforms and other proteins sequences will greatly aid our systematic understanding of the high flexibility of mandarin fish white muscle at molecular level and expand the utility of fish systems as models for the muscle genetic control and function. Springer Science+Business Media, LLC, 2010 Mandarin fish nationallicence ESTs nationallicence White muscle nationallicence Muscle protein nationallicence Isoforms nationallicence Zhang Guoqiang Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, People's Republic of China aut Chu Wuying Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut Hu Songnian Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, People's Republic of China aut Meng Tao Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut Pan Linlin Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, People's Republic of China aut Zhou Renxue Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut Liu Zhen Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut Zhang Jianshe Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 151-162 1436-2228 13:2<151 2011 13 10126 https://doi.org/10.1007/s10126-010-9275-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9275-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Guoqiang Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chu Wuying Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Hu Songnian Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Meng Tao Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Pan Linlin Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Renxue Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Liu Zhen Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jianshe Department of Bioengineering & Environmental Science, Changsha University, 410003, Changsha, People's Republic of China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 151-162 1436-2228 13:2<151 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer