caa a22 4500 445319003 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9274-2 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9274-2 Transgenic Expression of Walleye Dermal Sarcoma Virus rv-cyclin (orfA) in Zebrafish does not Result in Tissue Proliferation [Elektronische Daten] [Thomas Paul, Joel Rovnak, Sandra Quackenbush, Kathleen Whitlock, Huiqing Zhan, Zhiyuan Gong, Jan Spitsbergen, Paul Bowser, James Casey] Walleye dermal sarcoma (WDS) is a benign tumor of walleye fish that develops and completely regresses seasonally. The retrovirus associated with this disease, walleye dermal sarcoma virus, encodes three accessory genes, two of which, rv-cyclin (orfA) and orfb, are thought to play a role in tumor development. In this study, we attempted to recapitulate WDS development by expressing rv-cyclin in chimeric and stable transgenic zebrafish. Six stable transgenic lines expressing rv-cyclin from the constitutive CMVtk promoter were generated. Immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction demonstrate that rv-cyclin is widely expressed in different tissues in these fish. These lines were viable and histologically normal for up to 2years. No increase in tumors or tissue proliferation was observed following N-ethyl N-nitrosourea exposure or following tail wounding and subsequent tissue regeneration compared to controls. These data indicate that rv-cyclin is not independently sufficient for tumor induction in zebrafish. Springer Science+Business Media, LLC, 2010 Walleye dermal sarcoma virus nationallicence orfA nationallicence rv-cyclin nationallicence Fish retrovirus nationallicence Walleye dermal sarcoma nationallicence Zebrafish nationallicence Paul Thomas Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA aut Rovnak Joel Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA aut Quackenbush Sandra Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA aut Whitlock Kathleen Centro de Genómica de la Célula, Centro de Neurociencia, Universidad de Valparaíso, Valparaíso, Chile aut Zhan Huiqing Department of Biological Sciences, National University of Singapore, Singapore, Singapore aut Gong Zhiyuan Department of Biological Sciences, National University of Singapore, Singapore, Singapore aut Spitsbergen Jan Department of Microbiology and Marine and Freshwater Biomedical Sciences Center, Oregon State University, Corvallis, OR, USA aut Bowser Paul Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA aut Casey James Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 142-150 1436-2228 13:2<142 2011 13 10126 https://doi.org/10.1007/s10126-010-9274-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9274-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paul Thomas Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Rovnak Joel Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA aut NATIONALLICENCE 700 1- Quackenbush Sandra Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA aut NATIONALLICENCE 700 1- Whitlock Kathleen Centro de Genómica de la Célula, Centro de Neurociencia, Universidad de Valparaíso, Valparaíso, Chile aut NATIONALLICENCE 700 1- Zhan Huiqing Department of Biological Sciences, National University of Singapore, Singapore, Singapore aut NATIONALLICENCE 700 1- Gong Zhiyuan Department of Biological Sciences, National University of Singapore, Singapore, Singapore aut NATIONALLICENCE 700 1- Spitsbergen Jan Department of Microbiology and Marine and Freshwater Biomedical Sciences Center, Oregon State University, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Bowser Paul Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Casey James Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 142-150 1436-2228 13:2<142 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer