caa a22 4500 445319011 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9299-6 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9299-6 Different Pressure Resistance of Lactate Dehydrogenases from Hagfish is Dependent on Habitat Depth and Caused by Tetrameric Structure Dissociation [Elektronische Daten] [Yoshikazu Nishiguchi, Fumiyoshi Abe, Mitsumasa Okada] The effects of high hydrostatic pressure on lactate dehydrogenase (LDH) activities from two species of hagfish were examined. LDH from Eptatretus okinoseanus, a deep-sea species, retained 67% of the original activity even at 100MPa. LDH activity from Eptatretus burgeri, a shallow-sea species, was completely lost at 50MPa but recovered to the original value at 0.1MPa. The tetrameric structure of LDH-A4 from E. okinoseanus did not change at 50MPa. In contrast, almost all LDH tetramers from E. burgeri dissociated to dimers and monomers at 50MPa but reverted to tetramers at 0.1MPa. These results show that the dissociation of tetramers caused the inactivation of E. burgeri LDH. The difference depends on the number 6 and 10 amino acids. The mechanism of the slight, gradual inactivation of E. okinoseanus LDH at high pressure differs and is probably due to the metamorphosis of its inner structures. Springer Science+Business Media, LLC, 2010 Electrophoresis nationallicence Hagfish nationallicence Lactate dehydrogenase nationallicence Pressure resistance nationallicence Tetramer nationallicence Nishiguchi Yoshikazu Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, 274-8510, Funabashi, Japan aut Abe Fumiyoshi Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, 237-0061, Yokosuka, Japan aut Okada Mitsumasa Faculty of Science, Toho University, 2-2-1 Miyama, 274-8510, Funabashi, Japan aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 137-141 1436-2228 13:2<137 2011 13 10126 https://doi.org/10.1007/s10126-010-9299-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9299-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishiguchi Yoshikazu Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, 274-8510, Funabashi, Japan aut NATIONALLICENCE 700 1- Abe Fumiyoshi Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, 237-0061, Yokosuka, Japan aut NATIONALLICENCE 700 1- Okada Mitsumasa Faculty of Science, Toho University, 2-2-1 Miyama, 274-8510, Funabashi, Japan aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 137-141 1436-2228 13:2<137 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer