caa a22 4500 445319038 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9303-1 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9303-1 The Sesquiterpene Caulerpenyne from Caulerpa spp. is a Lipoxygenase Inhibitor [Elektronische Daten] [Sevilay Cengiz, Levent Cavas, Kadir Yurdakoc, Georg Pohnert] Many algae contain secondary metabolites with the potential to gain importance as pharmaceutically active secretions. Caulerpa racemosa var. cylindracea and Caulerpa prolifera are very abundant on the Mediterranean coastlines. The methanolic extracts of C. racemosa and C. prolifera were tested for inhibitory effects on soybean lipoxygenase. The extract of C. prolifera showed potent inhibitory effect in a lipoxygenase enzyme activity assay. HPLC comparison revealed that C. racemosa extract contained less caulerpenyne, the major secondary metabolite of both algae. In accordance with these findings, purified caulerpenyne inhibited lipoxygenase with an IC50 of 5.1μM. The enzyme kinetic studies indicated that both K M and V max decreased from 0.041 to 0.019mM and 312.5 to 151.5UmL−1 in the presence of 5µM caulerpenyne, revealing an un-competitive type of inhibition kinetics. The major secondary metabolite of Caulerpa species, caulerpenyne, is thus a novel lipoxygenase inhibitor that can be easily obtained in high quantities from the abundant algae. Springer Science+Business Media, LLC, 2010 Caulerpa prolifera nationallicence Caulerpa racemosa nationallicence Caulerpenyne nationallicence Lipoxygenase nationallicence Inhibitor nationallicence Cengiz Sevilay Faculty of Arts and Sciences, Department of Chemistry, Akdeniz University, Antalya, Turkey aut Cavas Levent Faculty of Arts and Sciences, Department of Chemistry, Dokuz Eylül University, Tinaztepe Campus, Izmir, Turkey aut Yurdakoc Kadir Faculty of Arts and Sciences, Department of Chemistry, Dokuz Eylül University, Tinaztepe Campus, Izmir, Turkey aut Pohnert Georg Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University of Jena, Jena, Germany aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 321-326 1436-2228 13:2<321 2011 13 10126 https://doi.org/10.1007/s10126-010-9303-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9303-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cengiz Sevilay Faculty of Arts and Sciences, Department of Chemistry, Akdeniz University, Antalya, Turkey aut NATIONALLICENCE 700 1- Cavas Levent Faculty of Arts and Sciences, Department of Chemistry, Dokuz Eylül University, Tinaztepe Campus, Izmir, Turkey aut NATIONALLICENCE 700 1- Yurdakoc Kadir Faculty of Arts and Sciences, Department of Chemistry, Dokuz Eylül University, Tinaztepe Campus, Izmir, Turkey aut NATIONALLICENCE 700 1- Pohnert Georg Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University of Jena, Jena, Germany aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 321-326 1436-2228 13:2<321 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer