caa a22 4500 445319046 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9285-z doi (NATIONALLICENCE)springer-10.1007/s10126-010-9285-z An Integrated Approach to Gene Discovery and Marker Development in Atlantic Cod ( Gadus morhua ) [Elektronische Daten] [Sharen Bowman, Sophie Hubert, Brent Higgins, Cynthia Stone, Jennifer Kimball, Tudor Borza, Jillian Bussey, Gary Simpson, Catherine Kozera, Bruce Curtis, Jennifer Hall, Tiago Hori, Charles Feng, Marlies Rise, Marije Booman, A. Gamperl, Edward Trippel, Jane Symonds, Stewart Johnson, Matthew Rise] Atlantic cod is a species that has been overexploited by the capture fishery. Programs to domesticate this species are underway in several countries, including Canada, to provide an alternative route for production. Selective breeding programs have been successfully applied in the domestication of other species, with genomics-based approaches used to augment conventional methods of animal production in recent years. Genomics tools, such as gene sequences and sets of variable markers, also have the potential to enhance and accelerate selective breeding programs in aquaculture, and to provide better monitoring tools to ensure that wild cod populations are well managed. We describe the generation of significant genomics resources for Atlantic cod through an integrated genomics/selective breeding approach. These include 158,877 expressed sequence tags (ESTs), a set of annotated putative transcripts and several thousand single nucleotide polymorphism markers that were developed from, and have been shown to be highly variable in, fish enrolled in two selective breeding programs. Our EST collection was generated from various tissues and life cycle stages. In some cases, tissues from which libraries were generated were isolated from fish exposed to stressors, including elevated temperature, or antigen stimulation (bacterial and viral) to enrich for transcripts that are involved in these response pathways. The genomics resources described here support the developing aquaculture industry, enabling the application of molecular markers within selective breeding programs. Marker sets should also find widespread application in fisheries management. The Author(s), 2010 Atlantic cod nationallicence ESTs nationallicence Markers nationallicence SNPs nationallicence Aquaculture nationallicence Bowman Sharen The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Hubert Sophie The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Higgins Brent The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Stone Cynthia The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Kimball Jennifer The Atlantic Genome Centre, NRC Institute for Marine Biosciences, National Research Council, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Borza Tudor The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Bussey Jillian The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Simpson Gary The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Kozera Catherine The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Curtis Bruce The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut Hall Jennifer Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut Hori Tiago Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut Feng Charles Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut Rise Marlies Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut Booman Marije Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut Gamperl A. Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut Trippel Edward Fisheries and Oceans Canada, St. Andrews Biological Station, 531 Brandy Cove Road, E5B 2L9, St. Andrews, NB, Canada aut Symonds Jane National Institute of Water and Atmospheric Research, NIWA Bream Bay Aquaculture Park, PO Box147, 0151, Ruakaka, New Zealand aut Johnson Stewart Pacific Biological Station, Fisheries and Oceans Canada, V9T 6N7, Nanaimo, BC, Canada aut Rise Matthew Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 242-255 1436-2228 13:2<242 2011 13 10126 https://doi.org/10.1007/s10126-010-9285-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9285-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bowman Sharen The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Hubert Sophie The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Higgins Brent The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Stone Cynthia The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Kimball Jennifer The Atlantic Genome Centre, NRC Institute for Marine Biosciences, National Research Council, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Borza Tudor The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Bussey Jillian The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Simpson Gary The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Kozera Catherine The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Curtis Bruce The Atlantic Genome Centre, NRC Institute for Marine Biosciences, 1411 Oxford Street, B3H 3Z1, Halifax, NS, Canada aut NATIONALLICENCE 700 1- Hall Jennifer Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut NATIONALLICENCE 700 1- Hori Tiago Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut NATIONALLICENCE 700 1- Feng Charles Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut NATIONALLICENCE 700 1- Rise Marlies Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut NATIONALLICENCE 700 1- Booman Marije Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut NATIONALLICENCE 700 1- Gamperl A. Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut NATIONALLICENCE 700 1- Trippel Edward Fisheries and Oceans Canada, St. Andrews Biological Station, 531 Brandy Cove Road, E5B 2L9, St. Andrews, NB, Canada aut NATIONALLICENCE 700 1- Symonds Jane National Institute of Water and Atmospheric Research, NIWA Bream Bay Aquaculture Park, PO Box147, 0151, Ruakaka, New Zealand aut NATIONALLICENCE 700 1- Johnson Stewart Pacific Biological Station, Fisheries and Oceans Canada, V9T 6N7, Nanaimo, BC, Canada aut NATIONALLICENCE 700 1- Rise Matthew Ocean Sciences Centre, Memorial University of Newfoundland, 1 Marine Lab Road, A1C 5S7, St. John's, NL, Canada aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 242-255 1436-2228 13:2<242 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer