caa a22 4500 445319062 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9281-3 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9281-3 Cloning of Exo-β-1,3-glucanase Gene from a Marine Yeast Williopsis saturnus and Its Overexpression in Yarrowia lipolytica [Elektronische Daten] [Ying Peng, Guang-Lei Liu, Xin-Jun Yu, Xiang-Hong Wang, Li Jing, Zhen-Ming Chi] The exo-β-1,3-glucanase structural gene (WsEXG1 gene, accession number: FJ875997.2) was isolated from both the genomic DNA and cDNA of the marine yeast Williopsis saturnus WC91-2 by inverse PCR and RT-PCR. An open reading frame of 1,254bp encoding a 417 amino acid protein (isoelectric point: 4.5) with calculated molecular weight of 46.2kDa was characterized. The promoter of the gene (intronless) was located from −28 to −77 and had one TATA box while its terminator contained the sequence AAGAACAATAAACAA from +1,386 to +1,401. The protein had the Family 5 glycoside hydrolase signature IGLELLNEPL and a fragment with the sequence of NLCGEWSAA, where the Glu-310 (E) was considered to be the catalytic nucleophile. The WsEXG1 gene was overexpressed in Yarrowia lipolytica Po1h and the recombinant WsEXG1 was purified and characterized. The molecular weight of the purified rWsEXG1 was 46.0kDa. The optimal pH and temperature of the purified rWsEXG1 were 5.0°C and 40°C, respectively. The purified rWsEXG1 had high exo-β-1,3-glucanase activity. Therefore, the recombinant β-1,3-glucanase may have highly potential applications in food and pharmaceutical industries. Springer Science+Business Media, LLC, 2010 Exo-β-1,3-glucanase nationallicence WsEXG1 gene nationallicence Williopsis saturnus nationallicence Marine yeasts nationallicence Y. lipolytica nationallicence Overexpression nationallicence Peng Ying UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut Liu Guang-Lei UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut Yu Xin-Jun UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut Wang Xiang-Hong UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut Jing Li UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut Chi Zhen-Ming UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 193-204 1436-2228 13:2<193 2011 13 10126 https://doi.org/10.1007/s10126-010-9281-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9281-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Ying UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut NATIONALLICENCE 700 1- Liu Guang-Lei UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut NATIONALLICENCE 700 1- Yu Xin-Jun UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut NATIONALLICENCE 700 1- Wang Xiang-Hong UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut NATIONALLICENCE 700 1- Jing Li UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut NATIONALLICENCE 700 1- Chi Zhen-Ming UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No.5, Qingdao, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 193-204 1436-2228 13:2<193 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer