caa a22 4500 445319070 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9283-1 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9283-1 Transcriptome Profiling of Embryonic Development Rate in Rainbow Trout Advanced Backcross Introgression Lines [Elektronische Daten] [Peng Xu, Lauren McIntyre, Julie Scardina, Paul Wheeler, Gary Thorgaard, Krista Nichols] In rainbow trout (Oncorhynchus mykiss) and other fishes, embryonic development rate is an ecologically and evolutionarily important trait that is closely associated with survival and physiological performance later in life. To identify genes differentially regulated in fast and slow-developing embryos of rainbow trout, we examined gene expression across developmental time points in rainbow trout embryos possessing alleles linked to a major quantitative trait loci (QTL) for fast versus slow embryonic development rate. Whole genome expression microarray analyses were conducted using embryos from a fourth generation backcross family, whereby each backcross generation involved the introgression of the fast-developing alleles for a major development rate QTL into a slow-developing clonal line of rainbow trout. Embryos were collected at 15, 19, and 28days post-fertilization; sex and QTL genotype were determined using molecular markers, and cDNA from 48 embryos were used for microarray analysis. A total of 183 features were identified with significant differences between embryonic development rate genotypes. Genes associated with cell cycle growth, muscle contraction and protein synthesis were expressed significantly higher in embryos with the fast-developing allele (Clearwater) than those with the slow-developing allele (Oregon State University), which may associate with fast growth and early body mass construction in embryo development. Across time points, individuals with the fast-developing QTL allele appeared to have earlier onset of these developmental processes when compared to individuals with the slow development alleles, even as early as 15days post-fertilization. Differentially expressed candidate genes chosen for linkage mapping were localized primarily to regions outside of the major embryonic development rate QTL, with the exception of a single gene (very low-density lipoprotein receptor precursor). Springer Science+Business Media, LLC, 2010 Rainbow trout nationallicence Gene expression nationallicence QTL nationallicence Embryonic development rate nationallicence Microarray nationallicence Xu Peng Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA aut McIntyre Lauren Department of Molecular Genetics and Microbiology, University of Florida, 32601, Gainesville, FL, USA aut Scardina Julie Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA aut Wheeler Paul School of Biological Sciences and Center for Reproductive Biology, Washington State University, 99164, Pullman, WA, USA aut Thorgaard Gary School of Biological Sciences and Center for Reproductive Biology, Washington State University, 99164, Pullman, WA, USA aut Nichols Krista Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 215-231 1436-2228 13:2<215 2011 13 10126 https://doi.org/10.1007/s10126-010-9283-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9283-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Peng Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA aut NATIONALLICENCE 700 1- McIntyre Lauren Department of Molecular Genetics and Microbiology, University of Florida, 32601, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Scardina Julie Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA aut NATIONALLICENCE 700 1- Wheeler Paul School of Biological Sciences and Center for Reproductive Biology, Washington State University, 99164, Pullman, WA, USA aut NATIONALLICENCE 700 1- Thorgaard Gary School of Biological Sciences and Center for Reproductive Biology, Washington State University, 99164, Pullman, WA, USA aut NATIONALLICENCE 700 1- Nichols Krista Department of Biological Sciences, Purdue University, 47907, West Lafayette, IN, USA aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 215-231 1436-2228 13:2<215 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer