caa a22 4500 445319089 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9295-x doi (NATIONALLICENCE)springer-10.1007/s10126-010-9295-x Exposure to dsRNA Elicits RNA Interference in Brachionus manjavacas (Rotifera) [Elektronische Daten] [Terry Snell, Tonya Shearer, Hilary Smith] RNA interference (RNAi) is a powerful technique for functional genomics, yet no studies have reported its successful application to zooplankton. Many zooplankton, particularly microscopic metazoans of phylum Rotifera, have unique life history traits for which genetic investigation has been limited. In this paper, we report the development of RNAi methods for rotifers, with the exogenous introduction of double-stranded RNA (dsRNA) through the use of a lipofection reagent. Transfection with dsRNA for heat shock protein 90, the membrane-associated progesterone receptor, and mitogen-activated protein kinase significantly increased the proportion of non-reproductive females. Additionally, a fluorescence-based lectin binding assay confirmed the significant suppression of four of six glycosylation enzymes that were targeted with dsRNA. Suppression of mRNA transcripts was confirmed with quantitative PCR. Development of RNAi for rotifers promises to enhance the ability for assessing genetic regulation of features critical to their life history and represents a key step toward functional genomics research in zooplankton. Springer Science+Business Media, LLC, 2010 RNA interference nationallicence Rotifera nationallicence Glycosylation nationallicence Quantitative PCR nationallicence Double-stranded RNA nationallicence Functional genomics nationallicence Snell Terry School of Biology, Georgia Institute of Technology, 311 Ferst Drive, 30332-0230, Atlanta, GA, USA aut Shearer Tonya School of Biology, Georgia Institute of Technology, 311 Ferst Drive, 30332-0230, Atlanta, GA, USA aut Smith Hilary School of Biology, Georgia Institute of Technology, 311 Ferst Drive, 30332-0230, Atlanta, GA, USA aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 264-274 1436-2228 13:2<264 2011 13 10126 https://doi.org/10.1007/s10126-010-9295-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9295-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Snell Terry School of Biology, Georgia Institute of Technology, 311 Ferst Drive, 30332-0230, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Shearer Tonya School of Biology, Georgia Institute of Technology, 311 Ferst Drive, 30332-0230, Atlanta, GA, USA aut NATIONALLICENCE 700 1- Smith Hilary School of Biology, Georgia Institute of Technology, 311 Ferst Drive, 30332-0230, Atlanta, GA, USA aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 264-274 1436-2228 13:2<264 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer