caa a22 4500 445319097 CHVBK 20180317142657.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9305-z doi (NATIONALLICENCE)springer-10.1007/s10126-010-9305-z Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish ( Danio rerio ) [Elektronische Daten] [Bo Guan, Hong Ma, Yaping Wang, Yuanlei Hu, Zhongping Lin, Zuoyan Zhu, Wei Hu] Aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp β-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F2 transgenic fish was significantly greater than that of the sibling controls under 2.5% O2 (dissolved oxygen (DO), 0.91mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish. Springer Science+Business Media, LLC, 2010 Vitreoscilla hemoglobin (VHb) nationallicence Hypoxia nationallicence Zebrafish ( Danio rerio ) nationallicence Transgenic fish nationallicence Guan Bo State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut Ma Hong College of Life Sciences, Peking University, 100871, Beijing, China aut Wang Yaping State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut Hu Yuanlei College of Life Sciences, Peking University, 100871, Beijing, China aut Lin Zhongping College of Life Sciences, Peking University, 100871, Beijing, China aut Zhu Zuoyan State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut Hu Wei State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 336-344 1436-2228 13:2<336 2011 13 10126 https://doi.org/10.1007/s10126-010-9305-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9305-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guan Bo State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Ma Hong College of Life Sciences, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Wang Yaping State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Hu Yuanlei College of Life Sciences, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Lin Zhongping College of Life Sciences, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Zhu Zuoyan State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Hu Wei State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 336-344 1436-2228 13:2<336 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer