caa a22 4500 445319119 CHVBK 20180317142658.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9282-2 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9282-2 The Effect of Ultrasonificated Extracts of Spirulina maxima on the Anticancer Activity [Elektronische Daten] [Sung-Ho Oh, Juhee Ahn, Do-Hyung Kang, Hyeon-Yong Lee] The effect of ultrasonic extraction on extraction yields, cytotoxicity, and anticancer activity of Spirulina maxima was investigated in this study. Optimal extraction conditions were determined as 60kHz frequency at 60°C for 30min with 120W intensity, which resulted in 19.3% of extraction yields and 19.1% of cytotoxicity on normal human cells. Yields from conventional water and ethanol extraction were 15.8% at 100°C and 8.3% at 80°C, respectively. It was found that the extracts obtained by ultrasonic extraction process selectively inhibited the digestive-related cancer cell lines, such as human stomach cancer cells, having 89% of the highest inhibition ratio and 4.5 of the highest selectivity. In adding 0.5mg/mL of the extract, human promyelocytic leukemia cells' cell differentiation was increased 1.72 times over that of the control. Expression level of B cell lymphoma-2 from Hep3B cell was also effectively suppressed by the extract obtained at 60kHz and 60°C, leading to the inhibition of the early step of carcinogenesis. This work suggests that anticancer activity of the extracts is due to water-soluble polysaccharides rather than proteins and is further supported by the result that the ultrasonification extraction process can efficiently extract relatively intact polysaccharides rather than digesting the proteins in S. maxima by matrix assisted laser desorption ionization-time of flight and high performance size exclusion chromatography chromatogram analyses. Therefore, ultrasonic extraction increases both extraction yield and the biological activity of S. maxima extracts, which might be useful as an alternative natural anticancer agent in the medical and food industries. Springer Science+Business Media, LLC, 2010 Spirulina maxima nationallicence Ultrasonification low-temperature extraction nationallicence Anticancer activity nationallicence Oh Sung-Ho College of Bioscience and Biotechnology Division of Biomaterials Engineering, Kangwon National University, 200-701, Chuncheon, South Korea aut Ahn Juhee College of Bioscience and Biotechnology Division of Biomaterials Engineering, Kangwon National University, 200-701, Chuncheon, South Korea aut Kang Do-Hyung Korea Ocean Research & Development Institute, Ansan, P.O. Box29, 426-744, Seoul, South Korea aut Lee Hyeon-Yong College of Bioscience and Biotechnology Division of Biomaterials Engineering, Kangwon National University, 200-701, Chuncheon, South Korea aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 205-214 1436-2228 13:2<205 2011 13 10126 https://doi.org/10.1007/s10126-010-9282-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9282-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oh Sung-Ho College of Bioscience and Biotechnology Division of Biomaterials Engineering, Kangwon National University, 200-701, Chuncheon, South Korea aut NATIONALLICENCE 700 1- Ahn Juhee College of Bioscience and Biotechnology Division of Biomaterials Engineering, Kangwon National University, 200-701, Chuncheon, South Korea aut NATIONALLICENCE 700 1- Kang Do-Hyung Korea Ocean Research & Development Institute, Ansan, P.O. Box29, 426-744, Seoul, South Korea aut NATIONALLICENCE 700 1- Lee Hyeon-Yong College of Bioscience and Biotechnology Division of Biomaterials Engineering, Kangwon National University, 200-701, Chuncheon, South Korea aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 205-214 1436-2228 13:2<205 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer