caa a22 4500 445319127 CHVBK 20180317142658.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9284-0 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9284-0 Comparative Genomics Identifies Candidate Genes for Infectious Salmon Anemia (ISA) Resistance in Atlantic Salmon ( Salmo salar ) [Elektronische Daten] [Jieying Li, Keith Boroevich, Ben Koop, William Davidson] Infectious salmon anemia (ISA) has been described as the hoof and mouth disease of salmon farming. ISA is caused by a lethal and highly communicable virus, which can have a major impact on salmon aquaculture, as demonstrated by an outbreak in Chile in 2007. A quantitative trait locus (QTL) for ISA resistance has been mapped to three microsatellite markers on linkage group (LG) 8 (Chr 15) on the Atlantic salmon genetic map. We identified bacterial artificial chromosome (BAC) clones and three fingerprint contigs from the Atlantic salmon physical map that contains these markers. We made use of the extensive BAC end sequence database to extend these contigs by chromosome walking and identified additional two markers in this region. The BAC end sequences were used to search for conserved synteny between this segment of LG8 and the fish genomes that have been sequenced. An examination of the genes in the syntenic segments of the tetraodon and medaka genomes identified candidates for association with ISA resistance in Atlantic salmon based on differential expression profiles from ISA challenges or on the putative biological functions of the proteins they encode. One gene in particular, HIV-EP2/MBP-2, caught our attention as it may influence the expression of several genes that have been implicated in the response to infection by infectious salmon anemia virus (ISAV). Therefore, we suggest that HIV-EP2/MBP-2 is a very strong candidate for the gene associated with the ISAV resistance QTL in Atlantic salmon and is worthy of further study. The Author(s), 2010 Infectious salmon anemia nationallicence Atlantic salmon nationallicence Disease resistance nationallicence Comparative genomics nationallicence Li Jieying Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, V5A 1S6, Burnaby, BC, Canada aut Boroevich Keith Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, V5A 1S6, Burnaby, BC, Canada aut Koop Ben Department of Biology, University of Victoria, V8W 3N5, Victoria, BC, Canada aut Davidson William Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, V5A 1S6, Burnaby, BC, Canada aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 232-241 1436-2228 13:2<232 2011 13 10126 https://doi.org/10.1007/s10126-010-9284-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9284-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jieying Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, V5A 1S6, Burnaby, BC, Canada aut NATIONALLICENCE 700 1- Boroevich Keith Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, V5A 1S6, Burnaby, BC, Canada aut NATIONALLICENCE 700 1- Koop Ben Department of Biology, University of Victoria, V8W 3N5, Victoria, BC, Canada aut NATIONALLICENCE 700 1- Davidson William Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, V5A 1S6, Burnaby, BC, Canada aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 232-241 1436-2228 13:2<232 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer