caa a22 4500 44531916X CHVBK 20180317142658.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10126-010-9304-0 doi (NATIONALLICENCE)springer-10.1007/s10126-010-9304-0 Crassostrea angulata Bindin Gene and the Divergence of Fucose-Binding Lectin Repeats Among Three Species of Crassostrea [Elektronische Daten] [Qi Wu, Li Li, Guofan Zhang] Bindin is a major protein for species-specific recognition between sperm and congenetic egg in many free-spawning marine invertebrates. We cloned a novel bindin gene from the oyster Crassostrea angulata by 3′ and 5′ rapid amplification of cDNA ends. The full-length bindin cDNA was 1,049bp with a 771-bp open reading frame encoding 257 amino acids. The deduced amino acid sequence contained a putative signal peptide of 24 amino acids. The length of the bindin genomic DNA was 8,508bp containing four exons and three introns. Three haplotypes of F-lectin repeat were detected from seven sequences of F-lectin repeat of six male oysters. Both neighbor-joining and minimum-evolution phylogenetic trees show that haplotype an1 was close to Crassostrea gigas while an2 and an3 were close to Crassostrea sikamea. Intron-4 in the middle of F-lectin repeat is highly variable in both size and sequence. We classified intron-4 into three types according to their size and the F-lectin repeat they were located in. Intron-4 may play an important role in recombination. We compared the number of nonsynonymous substitutions (Dn) and synonymous substitutions (Ds) per nucleotide site among 19 F-lectin haplotypes of the three species. Dn/Ds ratios suggested that positive selection occurred between C. gigas and C. sikamea and between C. gigas and C. angulata. Nine positive selected positions (p > 90%) are identified among 19 haplotypes of three species. They are located on the F-lectin binding face around the three recognition motif residues. We assume that these nine clustered amino acids are related with species-specific recognition. Springer Science+Business Media, LLC, 2010 Crassostrea angulata nationallicence Crassostrea nationallicence Bindin gene nationallicence cDNA cloning nationallicence Fucose-binding lectin nationallicence Positive selection nationallicence Wu Qi Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., 266071, Qingdao, China aut Li Li Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., 266071, Qingdao, China aut Zhang Guofan Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., 266071, Qingdao, China aut Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 327-335 1436-2228 13:2<327 2011 13 10126 https://doi.org/10.1007/s10126-010-9304-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10126-010-9304-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Qi Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., 266071, Qingdao, China aut NATIONALLICENCE 700 1- Li Li Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., 266071, Qingdao, China aut NATIONALLICENCE 700 1- Zhang Guofan Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., 266071, Qingdao, China aut NATIONALLICENCE 773 0- Marine Biotechnology Springer-Verlag 13/2(2011-04-01), 327-335 1436-2228 13:2<327 2011 13 10126 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer