caa a22 4500 44532113X CHVBK 20180317142704.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10534-010-9377-2 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9377-2 Cadmium-induced heme oxygenase-1 gene expression is associated with the depletion of glutathione in the roots of Medicago sativa [Elektronische Daten] [Weiti Cui, Guangqing Fu, Honghong Wu, Wenbiao Shen] Following previous findings that cadmium (Cd) induces heme oxygenase-1 (HO1) gene expression in alfalfa seedling roots, we now show that the decreased glutathione (GSH) and ascorbic acid (AsA) contents, induction of HO-1 gene expression and its protein level by Cd was mimicked by a GSH depletor diethylmaleate (DEM). Meanwhile, above Cd- or DEM-induced decreased GSH content followed by HO-1 up-regulation could be strengthened or reversed differentially by the application of a selective inhibitor of GSH biosynthesis l-buthionine-sulfoximine (BSO), or exogenous GSH and AsA, respectively. The antioxidative behavior of HO-1 induction was further confirmed by histochemical staining for the detection of loss of membrane integrity in a short period of treatment time. Additionally, the induction of HO-1 transcript was inhibited by the transcriptional inhibitor actinomycin D (ActD) or protein synthesis inhibitor cycloheximide (CX, especially). In contrast, the level of HO-2 transcript did not change upon various treatments. Together, above results suggested that Cd-induced up-regulation of HO-1 gene expression is associated with GSH depletion, which is at least existing transcriptional regulation level, thus leading to enhanced antioxidative capability transiently. Springer Science+Business Media, LLC., 2010 Alfalfa seedling roots nationallicence Ascorbic acid nationallicence Cd-induced oxidative stress nationallicence Glutathione depletion nationallicence HO - 1 gene expression nationallicence Cui Weiti College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Fu Guangqing College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Wu Honghong College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Shen Wenbiao College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut BioMetals Springer Netherlands 24/1(2011-02-01), 93-103 0966-0844 24:1<93 2011 24 10534 https://doi.org/10.1007/s10534-010-9377-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9377-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui Weiti College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Fu Guangqing College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Wu Honghong College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Shen Wenbiao College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/1(2011-02-01), 93-103 0966-0844 24:1<93 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer