caa a22 4500 445321164 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10534-010-9379-0 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9379-0 Protective effect of ascorbic acid on cadmium-induced hypertension and vascular dysfunction in mice [Elektronische Daten] [Wanida Donpunha, Upa Kukongviriyapan, Kwanjit Sompamit, Poungrat Pakdeechote, Veerapol Kukongviriyapan, Patchareewan Pannangpetch] Cadmium (Cd) is one of the most important environmental pollutants that cause a number of adverse health effects in humans and animals. Recent studies have shown that Cd-induced oxidative damage within the vascular tissues results in vascular dysfunction. The current study was aimed to investigate whether ascorbic acid could protect against Cd-induced vascular dysfunction in mice. Male ICR mice were received CdCl2 (100mg/l) via drinking water for 8weeks alone or received ascorbic acid supplementation at doses of 50 and 100mg/kg/day for every other day. Results showed that Cd administration increased arterial blood pressure and blunted the vascular responses to vasoactive agents. These alterations were related to increased superoxide production in thoracic aorta, increased urinary nitrate/nitrite, increased plasma protein carbonyl, elevated malondialdehyde (MDA) concentrations in plasma and tissues, decreased blood glutathione (GSH), and increased Cd contents in blood and tissues. Ascorbic acid dose-dependently normalized the blood pressure, improved vascular reactivities to acetylcholine (ACh), phenylephrine (Phe) and sodium nitroprusside (SNP). These improvements were associated with significant suppression of oxidant formation, prevention of GSH depletion, and partial reduction of Cd contents in blood and tissues. The findings in this study provide the first evidence in pharmacological effects of ascorbic acid on alleviation of oxidative damage and improvement of vascular function in a mouse model of Cd-induced hypertension and vascular dysfunction. Moreover, our study suggests that dietary supplementation of ascorbic acid may provide beneficial effects by reversing the oxidative stress and vascular dysfunction in Cd-induced toxicity. Springer Science+Business Media, LLC., 2010 Ascorbic acid nationallicence Cadmium nationallicence Hypertension nationallicence Oxidative stress nationallicence Vascular dysfunction nationallicence Donpunha Wanida Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Kukongviriyapan Upa Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Sompamit Kwanjit Faculty of Medicine, Mahasarakham University, 44150, Mahasarakham, Thailand aut Pakdeechote Poungrat Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Kukongviriyapan Veerapol Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Pannangpetch Patchareewan Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut BioMetals Springer Netherlands 24/1(2011-02-01), 105-115 0966-0844 24:1<105 2011 24 10534 https://doi.org/10.1007/s10534-010-9379-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9379-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donpunha Wanida Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Kukongviriyapan Upa Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Sompamit Kwanjit Faculty of Medicine, Mahasarakham University, 44150, Mahasarakham, Thailand aut NATIONALLICENCE 700 1- Pakdeechote Poungrat Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Kukongviriyapan Veerapol Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Pannangpetch Patchareewan Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/1(2011-02-01), 105-115 0966-0844 24:1<105 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer